[Antigenic preparations from Opisthorchis and their use in immunodiagnosis. 2. The effectiveness of antibody detection in infected patients].

Serological studies using opisthorchis immunoreagents of various specificity (metabolic, somatic and from eggs) have shown that metabolic antigen preparations were the most effective. Simultaneous use of 3 immunoreagents of various specificity ensures higher percentage of opisthorchiasis patients' identification. Passive hemagglutination test and enzyme immunoassay are practically identically effective for the antibody determination.

[Seroepidemiologic study of echinococcosis foci in southeastern Kazakhstan].

Mass screening of the southeastern Kazakhstan population with respect to echinococcosis by RDHA provided positive results in 3.1% cases. The highest seropositive reaction rate (6.

[Preparation and study of the efficacy of a toxoplasmic erythrocytic diagnostic agent].

Comparison of various antigenic preparations of Toxoplasma and of the methods for red cell sensitization with these antigens has shown that sensitization with Toxoplasma summary fraction, containing the cytoplasmic and envelope antigens, and rivanol, is the optimal method for preparing an erythrocytic diagnostic agent. A test with such a diagnosticum has proved to be much more effective than the complement fixation test. Though the results of the two tests correlate, this correlation is curvilinear, and therefore it is desirable to use both the tests for the diagnostic investigations.

[Use of enzyme-antibody immune complexes in serological analysis].

In model experiments with simulation of rabbit immune response to mouse IgG and antibody production in echinococcosis patients the indirect enzyme-linked immunosorbent assay has been several times more sensitive with peroxidase-antiperoxidase and catalase-anticatalase complexes than with enzyme-antiglobulin chemical conjugates. The immune complexes have been found to retain their activity for 6 months and longer.