[Structure of the oligosaccharide region (core) of the lipopolysaccharides of Shigella flexneri types 2a and 5b].

The full structure of the lipopolysaccharide core of bacteria Shigella flexneri types 2a and 5b, the causative agents of bacillary dysentery (shigellosis), was established by chemical methods, high-resolution electrospray ionization mass spectrometry, and two-dimensional NMR spectroscopy. The structure of the O-antigen repeating unit and the configuration and position of the linkage between the O-antigen and the core were determined in the lipopolysaccharide of S. flexneri type 2a.

[The structure of the O-specific polysaccharide from a mutant of nitrogen-fixing rhizobacterium Azospirillum brasilense Sp245 with an altered plasmid content].

The rhizobacteria Azospirillum brasilense Sp245 produce antigenically different lipopolysaccharides LPSI and LPSII, both containing identical pentasaccharides built from D-rhamnose residues as the repeated chains of O-specific oligosaccharides (OPS). In this study, we report the structure of the OPS from A. brasilense LPSI(-)LPSII(-)-mutant Sp245.

[Bacterial antigen polysaccharides. 43. Structure of O-specific polysaccharide of Providencia alcalifaciens O46].

Acid O-specific polysaccharide containing D-glucose, D-glucuronic acid, L-fucose, and 2-acetamido-2-deoxy-D-glucose was obtained by mild acid degradation of lipopolysaccharide from Providencia alcalifaciens O46. Consideration of the data revealed the following structure of the hexasaccharide repeating unit of O-specific polysaccharide under methylation analyses along with (1)H and (13)C NMR spectroscopy, including 2D (1)H, (1)H-COSY, TOCSY-, ROESY-,(1)H, (13)C-HSQC-, and HMQC-TOCSY experiments: [Formula: see text].

[Complete structure of the O-polysaccharide from Shigella dysenteriae type 10].

The structure of the O-specific polysaccharide from Shigella dysenteriae type 10, which has been reported previously in Bioorg. Khim. (1977, vol.

[Structures of O-polysaccharides from two Shigella dysenteriae type 8 strains].

The structure of the O-polysaccharide (O-antigen) from Shigella dysenteriae type 8 bacteria (strain 599) was corrected using modern NMR techniques (structure 1). The revisions concerned the position of the Glc residue (in the main, but not the side, chain), the site of its substitution, and the configuration of the O-glycoside linkage of the GlcNAc residue. The S.

[Antigenic polysaccharides of bacteria: 41. Structures of the O-specific polysaccharides of Shigella dysenteriae types 4 and 5 revised by NMR spectroscopy].

The earlier established structures of the acidic O-specific polysaccharides from two typical strains of the Shigella dysenteriae bacterium were revised using modern NMR spectroscopy techniques. In particular, the configurations of the glycosidic linkages of GlcNAc (S. dysenteriae type 4) and mannose (S.

[Antigenic polysaccharides of bacteria: 40. The structures of O-specific polysaccharides from Shigella dysenteriae types 3 and 9 and S. boydii type 4 revised by NMR spectroscopy].

The reported structures of O-specific polysaccharides from three standard strains of Shigella bacteria were corrected by modern NMR techniques. The revisions concerned the configuration of the O-glycoside linkage (S. dysenteriae type 3, structure 1), the positions of monosaccharide residue glycosylation and acetylation by pyruvic acid (S.

[The structure of the glycerophosphate-containing O-specific polysaccharide from Escherichia coli 0130].

A phosphorylated O-specific polysaccharide was obtained by mild acidic degradation of the lipopolysaccharide from the intestinal bacterium Escherichia coli 0130 and characterized by the methods of chemical analysis, including dephosphorylation, and 1H and 13C NMR spectroscopy. The polysaccharide was shown to be composed of branched tetrasaccharide repeating units containing two N-acetyl-D-galactosamine residues, D-galactose, D-glucose, and glycerophosphate residues (one of each). The polysaccharide has the following structure, which is unique among the known bacterial polysaccharides.

[Generation of vaccine strains of gram-negative bacteria with reduced adverse reactions].

The main shortcoming of the modem live and killed vaccines based on gram-negative bacterial strains is their ability to cause adverse reactions. The majority of the adverse reactions are associated with the effect of biological activity of lipopolysaccharide. The report covers the problems concerned with biogenesis of the lipid A, lipopolysaccharide structural component, responsible for its endotoxic activity, as well as with genes determining lipid A synthesis.

[Structural diversity of O-polysaccharides and serological classification of Pseudomonas syringae pv. garcae and other strains of genomospecies 4].

Novel O-serotypes were revealed among Pseudomonas syringae pv. garcae strains by using a set of mouse monoclonal antibodies specific to the lipopolysaccharide O-polysaccharide. Structural studies showed that the O-polysaccharide of P.

[Structure of O-specific polysaccharide from Proteus mirabilis O10, containing a new component of O-antigens--L-altruronic acid].

An acidic O-specific polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Proteus mirabilis O10 and studied after full acid hydrolysis and carboxyl reduction by 1H- and 13C-NMR spectroscopy, including two-dimensional correlation spectroscopy (COSY), H-detected heteronuclear 1H,13C multi-quantum coherence (HMQC), and rotating-frame nuclear Overhauser effect spectroscopy (ROESY). It was found that the polysaccharide contains 2-acetamido-2-deoxy-D-glucose, 2-acetamido-2-deoxy-D-galactose, D-galacturonic acid, and L-altruronic acid, and the following structure of the branched tetrasaccharide repeating unit of the polysaccharide was established: [sequence: see text]

[Structure of the O-specific polysaccharide chain from Burkholderia (Pseudomonas) solanacerum ICMP 750, 8093, 8115, 7864, and 7945 lipopolysaccharides].

Lipopolysaccharides (LPS) have been isolated from the cells of phytopathogenic bacteria Burkholderia (Pseudomonas) solanacearum strains (representatives of I, II and IV biovars) using mild acid hydrolysis followed by gel permeation chromatography of carbohydrate residues on Sephadex G-50 to yield five O-specific polysaccharides (O-PS). The O-PS structures were established by 13C-NMR spectroscopy. It has been found that O-PS have a linear or branched structure.

[Structure of the O-specific polysaccharide from Proteus mirabilis O30].

Based on acid hydrolysis, methylation, and one- and two-dimensional 1H- and 13C-NMR spectroscopy, including homonuclear and 1H, 13C heteronuclear correlation spectroscopy (COSY) and rotating-frame nuclear Overhauser effect spectroscopy (ROESY); it was found that the O-specific polysaccharide chain of the lipopolysaccharide of the bacterium Proteus mirabilis O30 is a hexosamino-glucuronan built up of tetrasaccharide repeating units having the following structure: [formula: see text] The degree of O-acetylation of 2-acetamido-2-deoxyglucose is about 70%.

[Structure of a new lysine-containing O-specific polysaccharide from Proteus mirabilis O26].

The composition and structure of the O-specific polysaccharide chain of the bacterium Proteus mirabilis O26 lipopolysaccharide have been studied using one- and two-dimensional 1H- and 13C-NMR spectroscopy, including homonuclear correlation spectroscopy (COSY), H-detected 1H,13C heteronuclear multi-quantum coherence and rotating-frame nuclear Overhauser effect spectroscopy. It has been found that the polysaccharide is acidic due to the presence of D-galacturonic acid (D-GalA) residues, part of which are O-acetylated, while the other part form an amide with the alpha-amino group of L-lysine and is built up of tetrasaccharide repeating units having the following structure: [formula: see text] The P. mirabilis O26 polysaccharide is structurally similar to the O-specific polysaccharide of P.

[Structure and properties of the common polysaccharide antigen of Pseudomonas aeruginosa bacteria (review)].

The review is devoted to a surface carbohydrate antigen of the bacterium Pseudomonas aeruginosa which is common for the majority of the species and has a lipopolysaccharide nature. The occurrence, detection, isolation, structure, expression on the cell surface, interaction with antibodies, an antibiotic and a bacteriophage as well as the immunotherapeutic potential of the common polysaccharide antigen are discussed.

[Structure of an acidic polysaccharide found in a bacteriolytic complex of lysoamidase].

A structural study of an acidic polysaccharide, a component of the lysoamidase bacteriolytic complex has been carried out. Analysis of the monosaccharide composition of the original polysaccharide, of the product of carboxyl groups reduction and of the 13C-NMR and 1H-NMR spectra of the original and the O-deacetylated polysaccharides using two-dimensional NMR spectroscopy has made it possible to establish the structure of the repeating trisaccharide unit of the polysaccharide as follows: [formula: see text] where ManNAcA and GalNAcA are 2-acetamido-2-deoxymannuronic acid and 2-acetamido-2-deoxygalacturonic acid, respectively. Also small amount of a neutral polysaccharide containing of rhamnose is present in the lysoamidase preparation.

[Structure of lipopolysaccharides from gram-negative bacteria. III. Structure of O-specific polysaccharides].

Data on the composition and structure of O-antigens which represent polysaccharide chains of outer membrane lipopolysaccharides of gram-negative bacteria defining cell immunospecificity are reviewed with special reference to some structural features of O-antigens, such as masked regularity and occurrence in one microorganism of lipopolysaccharides with two structurally distinct polysaccharide chains. Antigenic relationships between the microorganisms belonging to different taxonomic groups are discussed.

[Antigenic bacterial polysaccharides. Structure of O-specific polysaccharides from Pseudomonas cepacia serogroups C, I, O1, and O4].

Like some Pseudomonas cepacia serogroups studied earlier, serogroups C, I (Nakamura), O1 and O4 (Heidt) are characterized by the presence of at least two structurally different O-antigenic polysaccharide chains in cell-wall lipopolysaccharides. On the basis of acid hydrolysis, methylation, 1H- and 13C-NMR spectroscopy, including computer-assisted 13C-NMR-based analysis, the complete structures of the predominant polysaccharides of serogroups I (I), C and O4 (III) and the minor polysaccharides of serogroups I (II) and O1 (V) were established, and the structure of the predominant polysaccharide of serogroup O1 (IV) established earlier (Cox A. D.

[Structural study of O-specific polysaccharides from Proteus mirabilis O28 and 3/6, containing amides of D-galacturonic acid with L-amino acids; C-methylation and beta-elimination at L-serine and L-threonine residues upon analysis using the methylation method].

Structures of the O-specific polysaccharides of Proteus mirabilis O28 and 3/6 were studied by partial acid hydrolysis followed by borohydride reduction and methylation and GLC/MS analysis of the resulting glycosyl alditol derivatives. C-Methylation and beta-elimination in serine and threonine attached to the carboxyl group of galacturonic acid were observed in the course of the methylation analysis.

[Antigenic polysaccharides of bacteria 38. Structure of O-antigens of Pseudomonas solanacearum ICMP 7942 and ICMP 8169].

The structures I and II were established for the O-antigens of Pseudomonas solanacearum ICMP 7942 and ICMP 8169, respectively, by means of NMR-spectroscopy, including 2D homonuclear shift-correlated spectroscopy (COSY), rotating-frame NOE spectroscopy (ROESY) and heteronuclear 13C, 1H multiquantum correlation spectroscopy (HMQC). In the polysaccharide of the ICMP 8169 strain substitution of the main chain by the xylose residue is non-stoichiometric, i.e.

[Structure of the repeating unit of O-specific polysaccharide from Vibrio fluvialis].

O-Specific polysaccharide of Vibrio fluvialis, strain AQ-0002B, is built up to pentasaccharide repeating units contained of D-mannose, 2-acetamido-2-deoxy-D-glucose, 2-acetamido-2-deoxy-D-galactose, D-galacturonic acid and 3-0-[(R)-1'-carboxyethyl]-L-rhamnose (rhamnoactylic acid, Rha3Lac) residues. On the basis of methylation studies, solvolysis with HF, Smith degradation, 1H and 13C NMR-spectroscopy including homo- and heteronuclear correlation spectroscopy and NOE experiments, the following structure was suggested for the polysaccharide repeating unit: [formula: see text]

[Structure of the repeating unit of acid polysaccharide from Alteromonas macleodii 2MM6].

An acidic polysaccharide from Alteromonas macleodii 2MM6 is shown to consist of tetrasaccharide repeating units containing D-galactose, 3-O-acetyl-2-acetamido-2-deoxy-D-glucose, 2-acetamido-2-deoxy-L-guluronic acid and 3,6-dideoxy-3-(4-hydroxybutyramido)-D-galactose residues. On the basis of sugar analysis, 1H and 13C NMR-spectroscopy data including NOE and one-dimensional HOHAHA experiments, the following structure was suggested for the polysaccharide repeating unit: [formula: see text] R: -CO-CH2-CH2-CH2-OH.

[Structure of the repeating link of the acid polysaccharide of Alteromonas haloplanktis KMM 156].

An acidic capsular and an O-specific polysaccharide were isolated from the marine microorganism Alteromonas haloplanktis KMM 156. Both polysaccharides have the identical structure and are built up of tetrasaccharide repeating units, containing two residues of L-rhamnose as well as a 2-acetamido-2-deoxy-D-glucose and a 3-O-[(R)-1-carboxyethyl]-D-glucose (Glc3Lac) residue. On the basis of methylation studies, 1H- and 13C-NMR-spectroscopy including nuclear Overhauser effect and two-dimensional heteronuclear 13C/1H correlation spectroscopy, the following structure was suggested for the polysaccharide repeating unit: [formula: see text]

[Structure of lipopolysaccharides from gram-negative bacteria. II. Core structure (Review)].

The review summarizes the data on the core structure of bacterial lipopolysaccharides (LPS) which represents an oligosaccharide binding the lipid moiety of the macromolecule to the O-antigenic polysaccharide chain. Both S-strains with complete LPS and R-mutants having various defects of the core biosynthesis are considered. The role of the core in the outer membrane functioning and manifestation of antigenic specificity of LPS is discussed.