[About the impact of the dendritic cell autovaccine on the results of treatment of non-small-cell lung cancer patients].

In thoracic department of the National Cancer Institute studied the effectiveness of dendritic cell autovaccine in the postoperative period in non-small-cell lung cancer patients. The results, showing good tolerance dendritic cell autovaccine. Shows the formation of the expressed antigen immune response after repeated injections dendritic cell autovaccine, as manifested after 4 revaccination.

[Effect of dendritic cell autovaccine on the treatment outcome in patients with ovarian cancer].

During I/II phase clinical trial in ovarian cancer (OC) patients two types of autologous anticancer vaccines based on dendritic cells have been tested, and a comparative analysis of their effectiveness have been performed. It was shown that the anticancer vaccines based on DC, "loaded" with autologous tumor cell lysate obtained by treatment of tumor cells by cytotoxic lectins B. subtilis had higher efficiency, compared with the standard DC--autovaccine.

[Potential of prevention of metastasizing with the aid of tumor-specific transfer-factor].

The report discusses our experimental data in support of biotherapy which uses chemotherapy and antitumor immune treatment with in vivo xenogenic transfer-factor polypeptides (TFP) isolated from lymphocytes sensitized to antigens of given tumor. After excision of primary tumor--lung carcinoma of Lewis--mice C57BL/6 were injected intraperitoneally with xenogenic TFP (200 pg/body, twice) and a cytostaic dose of cyclophosphamide. Such adjuvant chemotherapy was found to prevent metastases from spreading to the lung in 100%.

[Transfer factor specific to mouse lung carcinoma of Lewis].

Factor of transfer was identified and sampled in the course of immunization of rats with cells or tissues of mouse lung carcinoma of Lewis and its antitumor action investigated on models of passive and spontaneous dissemination in mice C57BL/6. Samples obtained at the peak of immunological response (day 14) and immunological memory inception (day 60) were shown to be capable of marked antimetastatic effects: fall in metastatic frequency, number and size of metastases correlated with higher index of metastasis formation.

[Inhibition of tumor growth in rat liver induced by tumor-specific transfer factor].

A transfer factor (TF) specific to antigens of Geren's carcinoma in rat was developed using an original model of intraorganic growth. Intravenous injection (1pg/g body) inhibited primary tumor node growth in the liver by 78% and blocked dissemination to peritoneal viscera. Its effect was due to an immunospecific component promoting antitumor immunological response.

[Antitumor immunity transfer by a factor isolated from lymphocytes of tumor bearer].

Low-molecular extracts (LMEs) of lymphocytes were obtained from spleen of noninbred rats using our experimental model of intraorganic growth of Guerin's carcinoma. They were intended to transfer immune reaction to tumor antigens in vitro. It was LMEs developed prior to tumor progression in spleen that showed immunospecific activity with respect to tumor cells.

[Cytomorphological characteristics of dendritic cells].

Five morphological degrees of maturity of dendritic cells induced from monocytes of peripheral blood in oncological patients have been revealed using originally prepared and stained cytological preparations. Cytological examination of dendritic cells can be used for their identification in production of dendritic cell antitumor vaccines.

[Morphological changes in immunogenesis organs in transplantation of Guerin carcinoma to the spleen].

The study was made in 30 male Wistar rats (b.m. 110+/-10 g) with transplanted Guerin carcinoma to their spleen.

[Immune response in non-inbred rats after implantation of Geren carcinoma into their spleens].

Formation of specific immune response of Geren's carcinoma (GC) versus its biological properties was studied in non-inbred rat spleen. GC growth was accompanied by mounting immune response to tumor-associated antigens (TAA) which was evaluated by macrophage adherence inhibition test. Increase in cell-mediated response to such antigens passed through stages determined by the peculiarities of GC growth in spleen tissues.

[Changes in immunogenesis organs after thyroidectomy and hormonal correction in an experiment].

Regulating effect of thyroid hormones on functions of organs of the immune system has been demonstrated in the experiment. Deficiency of thyroid hormones affects thymulin synthesis and morphofunctional status of immunogenesis organs. Combination of thymostimulin and thyroxin in the thyroidectomized animals, as compared to their separate use, is pathogenetically the most adequate correcting therapy aiming normalization of structural and functional disorders in the thymus and spleen.

[Correlated connection of endocrine function of thymus and other internal secretion glands at stages of developing uterine choriocarcinoma].

The mechanisms of endocrine and immune system disorders in normal and disturbed pregnancy as well as in chorionocarcinoma, hydatid mole, metastatic and non-metastatic chorionocarcinoma have been investigated. Relevant disorders were chiefly caused by the endocrine function of the thymus and gonadotropins from the trophoblast, which made a powerful impact on the periphery of hormonal and immunological homeostasis. Unlike normal pregnancy, gestational trophoblastic disease involved changes in the nature, direction and degree of correlations between endocrine formations, which in turn determined the pathogenetic pattern of the disease.

[A comparison of the morphofunctional characteristics of the peripheral blood lymphocytes and the immune status of patients with malignant melanoma of the skin].

As a results of the study of immune status of 75 patients with malignant skin melanoma, some signs of immunodeficiency with failure of cell and humoral reactions were revealed. The degree of quantitative and qualitative changes of immune system depended on clinical-morphological features of malignancy development.

[The level of embryonic antigens in the blood serum and cerebrospinal fluid of neuro-oncological patients].

The levels of embryonic antigens (cancer embryonic antigen, alpha-fetoprotein, beta-chorionic gonadotropin were measured in the serum, spinal fluid, the cystic fluid of tumor 83 of neuro-oncological patients. It was found that the blood concentrations of cancer embryonic antigen and alpha-fetoprotein were not generally related to the degree of malignancy and the blood level of beta-chorionic gonadotropin decreased with the higher degree of tumor anaplasia. The high content of beta-chorionic gonadotropin in the spinal fluid suggests its origin from a brain tumor.

[The lymphokine-mediated activation of effector cells in the natural resistance system induced by gamma-interferon].

Some mechanisms of biologic action of gamma-interferon (INF) agents have been studied in mice systems in vivo. The obtained results testify to the possibility of existence of inductor mechanisms for activation of the effector cells of immunobiologic surveillance. It is supposed that gamma-INF is a binding chain of the natural host resistance.

[The mechanism of the immunomodulating action of Béres Drops Plus].

Béres Drops Plus were found to raise the number of E-RFCs, being formed by lymphocytes of peripheral blood of subjects presenting with low levels of T-cells, and to increase the production of substances possessing thymosine-like activity (STLA) by lymphocytes and epithelial cells of the murine thymus gland. In vitro the drug preparation in question enhances endocrine function of thymus in normal animals and the organism's capacity to induce STLA synthesis following thymectomy. These findings are useful in devising rational schemes of rehabilitation of immune system with the drug preparation Béres Drops Plus in secondary immunodeficiency states developing in cases presenting with precancer conditions, malignant neoplasia, in those subjects having taken part in the elimination of the Chernobyl NPP accident aftermath, those residing on the territories controlled, as well as evolving on account of aging.

[Endocrine function of the thymus and ionizing radiation].

It is shown that external as well as incorporated radiation inhibit the endocrine function of the thymus in the result of the direct and indirect effects. Postradiation deficiency of thymic hormones plays a significant role in pathogenesis of functional disorders in the immunity system. Immunocorrection with thymic agents is the most effective method when the mechanism of inhibition of thymic hormonal function are taken into account.

[Lymphocyte mechanoluminescence].

A unique installation and an original investigation method are described for studying mechanoluminescence of lymphocytes. The spectral content of mechanoluminescence of lymphocytes of human peripheral blood showed maxima of optical emission at 262, 291, 335 and 412 nm. Intraperitoneal administration of hydrocortisone in mice enhances thymocyte mechanoluminescence intensity after 2 and 3 days by 33 and 62%, resp.

[Changes in the neuroendocrine and immune state of adaptational homeostasis in normal pregnancy].

43 women with physiologically proceeding pregnancy have been examined during 1st, 2nd and 3d terms. The control group consists of 28 practically healthy non-pregnant women. It is stated that during pregnancy essential changes in the function of endocrine glands of mother take place.

[Effectiveness of correcting radiation injuries of the thymic endocrine function by T-activin in conditions of reduced postradiation hypercorticism reaction].

T-activin administered to rats after exposure to whole-body 1.5 Gy neutron- and 6 Gy X-radiation increases considerably the thymosin-like serum activity, accelerates cellularity restoration in the thymus and spleen, but does not influence the survival rate. Ionol administered prior to X-irradiation reduces the postirradiation hypercorticism reaction and the indirect effect of radiation on lymphoid organs which it is responsible for.

[Thymus endocrine function and the immune system indices of cancer patients after neutron and gamma therapy].

Patients with head and neck and locomotor system tumors received neutron therapy in the total doses of 4-8 and 12-14 Gy which was followed by a pronounced dose-dependent decrease in the serum thymus factor and total blood-lymphocyte levels. The latter changes were predominantly due to a decrease in the non-T-non-B cell concentration. Following the treatment, a rise in the level of circulating immune complexes and those of IgA and IgG was observed.

[Status of the endocrine function of the thymus gland in the normal and complicated course of pregnancy].

A total of 84 women with normal pregnancy and those with threatened abortions were examined, as were 28 healthy nonpregnant women, to estimate the function of the thymus. The findings evidence enhancement of the endocrine function of the thymus in pregnancy, this function being assessed from the thymic serum factor titer. The function activation is related to pregnancy course and is more marked in threatened abortion.

[The possibility of the restoration of interferon formation by biologically active thymus factors in experimentally induced immunodepression].

Interferon production was investigated in mice of CBA line with experimentally induced immunosuppression. With all types of treatment (irradiation, administration of hydrocortisone, cyclophosphane, ALS) the capacity of host cells for interferon production was shown to be reduced. Administration of biologically active thymus factor, thymostimulin, to experimental animals resulted in significant restoration of alpha/beta and gamma interferon production.

[The status of the endocrine function of the thymus in acute radiation sickness resulting from the accident at the Chernobyl Nuclear Power Station].

Data are presented on the role of inhibition of endocrine function of the thymus in the pathogenesis of acute radiation sickness resulted from the direct and indirect (via the increased glucocorticoid production) effects of ionizing radiation. The complex treatment, including nonspecific active immunotherapy, permitted to normalize the thymic hormone level and certain parameters of the immune system.