Nitrosopersulfide (SSNO) Is a Unique Cysteine Polysulfidating Agent with Reduction-Resistant Bioactivity.

The aim of the present study was to investigate the biochemical properties of nitrosopersulfide (SSNO), a key intermediate of the nitric oxide (NO)/sulfide cross talk. We obtained corroborating evidence that SSNO is indeed a major product of the reaction of S-nitrosothiols with hydrogen sulfide (HS). It was found to be relatively stable (t ∼1 h at room temperature) in aqueous solution of physiological pH, stabilized by the presence of excess sulfide and resistant toward reduction by other thiols.

The fluorescent dye 3,3'-diethylthiatricarbocyanine iodide is unsuitable for in vivo imaging of myelination in the mouse.

There is a growing interest to use non-invasive optical imaging methods to study central nervous system diseases. The application of a myelin-binding fluorescent dye, 3,3-diethylthiatricarbocyanine iodide (DBT) was recently described for in vivo optical imaging of demyelination in the mouse. In the present study we aimed at adapting the method to our optical imaging systems, the IVIS Lumina II to measure epifluorescence and the fluorescent molecular tomograph (FMT) for 3-dimensional quantification of the fluorophore.

Effects of sulfide and polysulfides transmitted by direct or signal transduction-mediated activation of TRPA1 channels.

Hydrogen sulfide (H S) is a gaseous mediator in various physiological and pathological processes, including neuroimmune modulation, metabolic pathways, cardiovascular system, tumour growth, inflammation and pain. Now the hydrogen polysulfides (H S ) have been recognised as signalling molecules modulating ion channels, transcription factors and protein kinases. Transient receptor potential (TRP) cation channels can be activated by mechanical, thermal or chemical triggers.

Atropine and glycopyrrolate do not support bacterial growth-safety and economic considerations.

Study Objective: Evaluation of bacterial growth in atropine and glycopyrrolate.

Design: Laboratory investigation.

Subjects And Measurements: Standard microbiological methods were used to evaluate the impact of atropine and glycopyrrolate on the growth of Acinetobacter baumannii, Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli.