During the total solar eclipses on 11 August 1999 in Kecel (Hungary) and on 29 March 2006 in Side (Turkey), two Hungarian groups performed full-sky imaging polarimetric measurements of the eclipsed sky. They observed the spatiotemporal change of the celestial polarization pattern and detected three polarization neutral points as well as two points with maximal polarization of the sky. Parallel to these studies, the polarization pattern in front of the lunar disc, the solar corona, and the surrounding sky have also been measured.
View Article and Find Full Text PDFBetween AD 900 and AD 1200 Vikings, being able to navigate skillfully across the open sea, were the dominant seafarers of the North Atlantic. When the Sun was shining, geographical north could be determined with a special sundial. However, how the Vikings could have navigated in cloudy or foggy situations, when the Sun's disc was unusable, is still not fully known.
View Article and Find Full Text PDFAmyloids are highly organized insoluble protein aggregates that are associated with a large variety of degenerative diseases. In this work, we investigated the anisotropic architecture of isolated human amyloid samples stained with Congo Red. This was performed by fluorescence detected linear dichroism (FDLD) imaging in a laser scanning confocal microscope that was equipped with a differential polarization attachment using high frequency modulation of the polarization state of the laser beam and a demodulation circuit.
View Article and Find Full Text PDFWe have constructed differential polarization (DP) attachments to a laser scanning microscope (LSM) for imaging the main DP quantities of anisotropic microscopic objects. The DP-LSM operates with high-frequency modulation and subsequent demodulation and displays the main DP quantities pixel by pixel. These, for linearly polarized light, include: (i) linear birefringence (LB), which is exhibited by structurally and/or optically anisotropic material; (ii) linear dichroism (LD), which carries information on the anisotropic distribution of the molecules, i.
View Article and Find Full Text PDFAnisotropy carries important information on the molecular organization of biological samples. Its determination requires a combination of microscopy and polarization spectroscopy tools. The authors constructed differential polarization (DP) attachments to a laser scanning microscope in order to determine physical quantities related to the anisotropic distribution of molecules in microscopic samples; here the authors focus on fluorescence-detected linear dichroism (FDLD).
View Article and Find Full Text PDFLipid rafts are cholesterol- and glycosphingolipid-rich plasma membrane microdomains, which control signal transduction, cellular contacts, pathogen recognition, and internalization processes. Their stability/lifetime, heterogeneity remained still controversial, mostly due to the high diversity of raft markers and cellular models. The correspondence of the rafts of living cells to liquid ordered (Lo) domains of model membranes and the effect of modulating rafts on the structural dynamics of their bulk membrane environment are also yet unresolved questions.
View Article and Find Full Text PDFNull-mutation in Drosophila importin-alpha2, such as the deficiency imp-alpha2(D14), causes recessive female sterility with the formation of dumpless eggs. In imp-alpha2(D14) the transfer of nurse cell components to the oocyte is interrupted and the Kelch protein, an oligomeric ring canal actin organizer, is normally produced but fails to associate with the ring canals resulting in their occlusion. To define domains regulating Kelch deposition on ring canals we performed site-directed mutagenesis on protein binding domains and putative phosphorylation sites of Imp-alpha2.
View Article and Find Full Text PDFThe optical alignment of biological samples is of great relevance to microspectrometry and to the micromanipulation of single particles. Recently, Bayoudh et al. (J.
View Article and Find Full Text PDFWe report here on the observation of unpolarized (neutral) points in the sky during the total solar eclipse on 11 August 1999. Near the zenith a neutral point was observed at 450 nm at two different points of time during totality. Around this celestial point the distribution of the angle of polarization was heterogeneous: The electric field vectors on the one side were approximately perpendicular to those on the other side.
View Article and Find Full Text PDFIn bird experiments which investigate the role of optical orientation cues associated with sunset it is a common method to reflect the celestial sunset factors by vertical deflector panels. Similar panels are also used in the deflector loft of homing pigeons that rotate wind and light cues in a clockwise or counterclockwise direction. The responses of test birds in these behavioural experiments often appear to be contradictory, because the deflectors produce a polarized optical stimulus that differs both qualitatively and quantitatively from the natural skylight polarization.
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