Dual-Functionalized Zwitterionic Polymers for Cell Cryopreservation.

Cryopreservation is an essential technique for the long-term preservation of cells. Some cells are challenging to cryopreserve, and novel cryoprotective agents are required. We previously reported a zwitterionic polymer (poly(ZI)) as a cryoprotectant that forms a polymer matrix surrounding the cells and partially prevents intracellular ice formation.

Optimization of Zwitterionic Polymers for Cell Cryopreservation.

Cryopreservation techniques are valuable for the preservation of genetic properties in cells, and the development of this technology contributes to various fields. In a previous study, an isotonic freezing medium composed of poly(zwitterion) (polyZI) has been reported, which alleviates osmotic shock, unlike typical hypertonic freezing media. In this study, the primitive freezing medium composed of emerging polyZI is optimized.