Chondrocyte viability in press-fit cryopreserved osteochondral allografts.

The viability of chondrocytes in press-fit glycerol-preserved osteochondral allografts was compared to that in fresh autografts, after transplantation into load-bearing and non-load-bearing sites in mature sheep stifle joints. We used macroscopic grading, tonometer pen indentation testing, histology, sulfate uptake and viability as determined by confocal-microscopy to assess cartilage condition. Despite there being no statistical differences between macroscopic appearance and tonometer testing of all grafts, confocal microscopy and histology demonstrated a positive effect of load-bearing placement on cryopreserved osteochondral allografts.

Chemical and biomechanical characterization of hyperhomocysteinemic bone disease in an animal model.

Background: Classical homocystinuria is an autosomal recessive disorder caused by cystathionine beta-synthase (CBS) deficiency and characterized by distinctive alterations of bone growth and skeletal development. Skeletal changes include a reduction in bone density, making it a potentially attractive model for the study of idiopathic osteoporosis.

Methods: To investigate this aspect of hyperhomocysteinemia, we supplemented developing chicks (n = 8) with 0.

Changes in human knee ligament stiffness secondary to osteoarthritis.

Stiffness of the medial (MCL) and lateral (LCL) collateral ligaments was compared between a group of 10 patients undergoing total knee arthroplasty for varus degenerative osteoarthritis (OAP), a group of 10 osteoarthritic cadaveric knees (OAC), and a group of 10 non-osteoarthritic cadaveric knees (NOA). A load-elongation curve was obtained for the medial and lateral compartments of each knee using an instrumented Moreland spreader. A strain gage (SG) was attached to the spreader handle and strain was calibrated to load applied against the spread distance.

Combined models of ultrasound velocity and attenuation for predicting trabecular bone strength and mineral density.

Three quantitative ultrasound parameters and their combinations were examined as possible predictors of the bone mineral density and compressive strength of human cancellous bone. Seventy-three trabecular bone cubes obtained from female cadaveric tibiae were measured by quantitative computed tomography. Measurements of the ultrasound velocity, ultrasound attenuation at 0.