Capture and X-ray diffraction studies of protein microcrystals in a microfluidic trap array.

X-ray free-electron lasers (XFELs) promise to enable the collection of interpretable diffraction data from samples that are refractory to data collection at synchrotron sources. At present, however, more efficient sample-delivery methods that minimize the consumption of microcrystalline material are needed to allow the application of XFEL sources to a wide range of challenging structural targets of biological importance. Here, a microfluidic chip is presented in which microcrystals can be captured at fixed, addressable points in a trap array from a small volume (<10 µl) of a pre-existing slurry grown off-chip.

An implantable microfluidic device for self-monitoring of intraocular pressure.

Glaucoma is the second most common cause of blindness in the world. It is a multifactorial disease with several risk factors, of which intraocular pressure (IOP) is a primary contributing factor. IOP measurements are used for glaucoma diagnosis and patient monitoring.

Recent developments in microfluidic large scale integration.

In 2002, Thorsen et al. integrated thousands of micromechanical valves on a single microfluidic chip and demonstrated that the control of the fluidic networks can be simplified through multiplexors [1]. This enabled realization of highly parallel and automated fluidic processes with substantial sample economy advantage.

Microfluidic very large scale integration (mVLSI) with integrated micromechanical valves.

Microfluidic chips with a high density of control elements are required to improve device performance parameters, such as throughput, sensitivity and dynamic range. In order to realize robust and accessible high-density microfluidic chips, we have fabricated a monolithic PDMS valve architecture with three layers, replacing the commonly used two-layer design. The design is realized through multi-layer soft lithography techniques, making it low cost and easy to fabricate.

Nanoamorphous carbon as a blackbody source in plasmonic thermal emitters.

Ag/dielectric/Ag-type plasmonic thermal emitters (PTEs) following a hexagonal lattice were fabricated, and their plasmonic emission spectrums were characterized with Fourier transform infrared spectroscopy. Nanoamorphous carbon (NAC) is used as a dielectric layer. Doping NAC with various materials over a wide range of levels enables control of the resistivity of the composite films where MoSi(2) was selected as the dopant.

Alignment-free fabrication of a hybrid electro-optic polymer/ion-exchange glass coplanar modulator.

A hybrid electro-optic (EO) polymer phase modulator with a 6 μm coplanar electrode gap was realized on ion exchange glass substrates. The critical alignment steps which may be required for hybrid optoelectronic devices were eliminated with a simple alignment-free fabrication technique. The low loss adiabatic transition from glass to EO polymer waveguide was enabled by gray scale patterning of novel EO polymer, AJLY.

Highly sensitive spectroscopic detection of heme-protein submonolayer films by channel integrated optical waveguide.

A highly sensitive technique based on optical absorption using a single-mode, channel integrated optical waveguide is described for broad spectral band detection and analysis of heme-containing protein films at a glass/water interface. Fabrication steps and device characteristics of optical waveguides suitable for operation in the wavelength range of 400 - 650 nm are described. Experimental results reported here show a limit of detection smaller than 100 pg/cm(2) for a submonolayer of ferricytochrome c by probing the Soret transition band with a 406-nm semiconductor diode laser propagating in a single-mode, ion-exchanged channel waveguide.