Black fly diversity and molecular detection of blood parasites in Simulium khelangense (Diptera, Simuliidae) from Laos.

Black flies (Diptera: Simuliidae) are significant pests and vectors transmitting pathogens to humans and other animals. However, knowledge of species diversity as well as their role as pests and vectors in Laos is limited, despite the country having abundant suitable lotic habitats, that is, running water. In this study, we collected wild adult black fly specimens from Laos.

Discovery of the Larvae and Pupae of the Black Fly () and Breeding Habitats of Potential Pest Species of the . (.) Subgroup (Simuliidae).

Two species of black flies (Simuliidae) in Thailand, Takaoka and Kuvangkadilok, 2000, and Takaoka, Srisuka & Saeung, 2022, are potent vectors of avian blood protozoa of the genera and and are pests of domestic avian species. Although the adults are abundant throughout Thailand, information on their breeding habitats is limited, and the immature stages of are unknown. We collected the larvae and pupae of from the Mekong River, the first-ever record of Simuliidae from this large continental river.

Molecular detection of blood protozoa and identification of black flies of the Simulium varicorne species group (Diptera: Simuliidae) in Thailand.

Species of the Simulium varicorne group in Thailand have veterinary significance as vectors of haemosporidian parasites. Accurate identification is, therefore, critical to the study of vectors and parasites. We used morphology and molecular markers to investigate cryptic genetic lineages in samples identified as Simulium chumpornense Takaoka & Kuvangkadilok, 2000.

Preconcentration of Heterocyclic Aromatic Amines in Edible Fried Insects Using Surfactant-Assisted Hydrophobic Deep Eutectic Solvent for Homogeneous Liquid-Liquid Microextraction prior to HPLC.

Thermal processing techniques are often accompanied by the production of many harmful compounds such as heterocyclic aromatic amines (HAAs). To protect human health, an efficient and environmentally friendly method, namely, homogeneous liquid-liquid microextraction (HLLME), was investigated. This method is based on a surfactant-assisted hydrophobic deep eutectic solvent for the determination of HAAs in edible fried insect samples prior to their analysis by high-performance liquid chromatography coupled with UV detection.

Diversity, Distribution and Host Blood Meal Analysis of Adult Black Flies (Diptera: Simuliidae) from Thailand.

Understanding the factors associated with the species diversity and distribution of insect vectors is critically important for disease epidemiology. Black flies (Diptera: Simuliidae) are significant hematophagous insects, as many species are pests and vectors that transmit pathogens to humans and other animals. Ecological factors associated with black fly species distribution have been extensively examined for the immature stages but are far less well explored for the adult stage.

Diversity, Abundance and Host Blood Meal Analysis of Latreille (Diptera: Ceratopogonidae) from Cattle Pens in Different Land Use Types from Thailand.

Biting midges of the genus Latreille are significant pests and vectors that transmit pathogens to humans and other animals. Cattle are among the important livestock that can potentially be severely affected by . In this study, we examined the species diversity, abundance, and host blood meal identification of biting midges in cattle pens located in three different land use types: villages, agricultural areas, and the forest edge.