Publications by authors named "Isao Terado"
Determination of free N-acetylneuraminic acid in urine by high-performance liquid chromatography using 3-[(1-[[4-(5,6-dimethoxy-1-oxoisoindolin-2-yl)-2-methoxyphenyl]sulfonyl]pyrrolidin-2-yl)carbonylamino]phenylboronic acid as a fluorescent labeling reagent.
J Chromatogr B Analyt Technol Biomed Life Sci
May 2003
Article Synopsis
- A sensitive HPLC method was developed to measure urinary N-acetylneuraminic acid (NeuAc) using a specific fluorescent labeling reagent.
- The reaction for labeling NeuAc was conducted at 30°C for 30 minutes and monitored at specific wavelengths.
- The method showed a detection limit of 48 fmol per injection, very low relative standard deviations for precision, and successfully analyzed diluted urine samples with concentrations ranging from 8 to 89 nmol/mg of creatinine.