Publications by authors named "Isabell Keil"
Article Synopsis
- Lipid nanoparticles (LNPs) are currently the best method for delivering messenger RNA (mRNA), and a new, cost-effective microfluidic setup has been developed for their synthesis.
- This new method allows for precise control of LNP characteristics and shows better structural uniformity compared to LNPs made with commercial equipment or ethanol injection.
- While the manufacturing process impacts the LNP's structure, it has only a minor effect on how effectively mRNA is delivered both in lab settings and in living organisms.
Lipid nanoparticles (LNPs) have gained great attention as carriers for mRNA-based therapeutics, finding applications in various indications, extending beyond their recent use in vaccines for infectious diseases. However, many aspects of LNP structure and their effects on efficacy are not well characterized. To further exploit the potential of mRNA therapeutics, better control of the relationship between LNP formulation composition with internal structure and transfection efficiency in vitro is necessary.
View Article and Find Full Text PDFmRNA based infectious disease vaccines have opened the venue for development of novel nucleic acids-based therapeutics. For all mRNA therapeutics dedicated delivery systems are required, where different functionalities and targeting abilities need to be optimized for the respective applications. One option for advanced formulations with tailored properties are lipid-polymer hybrid nanoparticles with complex nanostructure, which allow to combine features of several already well described nucleic acid delivery systems.
View Article and Find Full Text PDFBackground: RNA-based vaccination strategies tailoring immune response to specific reactions have become an important pillar for a broad range of applications. Recently, the use of lipid-based nanoparticles opened the possibility to deliver RNA to specific sites within the body, overcoming the limitation of rapid degradation in the bloodstream. Here, we have investigated whether small animal PET/MRI can be employed to image the biodistribution of RNA-encoded protein.
View Article and Find Full Text PDFObjective: To compare bone conduction after 2 different ways of perforating the stapes footplate during stapedotomy in patients with otosclerosis.
Study Design: Retrospective clinical study.
Setting: Tertiary reference center.