High-throughput rapid amplicon sequencing for multilocus sequence typing of from archived clinical DNA samples.

Introduction: Spillover events of have devastating effects on the wild sheep populations. Multilocus sequence typing (MLST) is used to monitor spillover events and the spread of between the sheep populations. Most studies involving the typing of have used Sanger sequencing.

Complete genome sequences of and isolated from dogs and cats with infectious respiratory disease.

is a primary agent of pneumonia in dogs, and is associated with upper respiratory tract disease in cats. We present complete genome sequences of 26 isolates from clinically affected dogs and cats. These genome sequences will facilitate new molecular and epidemiological analyses.

Development of a long-read next generation sequencing workflow for improved characterization of fastidious respiratory mycoplasmas.

and are often associated with canine and feline infectious respiratory disease in dogs and cats, respectively. Mycoplasmas have a reduced genome and dearth of many biosynthetic pathways, making them dependent on rich medium for growth. Due to this fastidious nature, mycoplasmas have been historically underdiagnosed.