Long COVID Following Mild SARS-CoV-2 Infection: A Retrospective Study in a Portuguese Primary Health Care Unit.

Introduction: Long COVID is a recent pathological entity. Its manifestations and impact on health and quality of life and, on a larger scale, at an economic and social level may be very significant, reflecting a challenge for the future. The family doctor plays a major role in the care of these patients and must be aware of this new reality.

Proteomics Analysis of Ovarian Cancer Cell Lines and Tissues Reveals Drug Resistance-associated Proteins.

Background: Carboplatin and paclitaxel form the cornerstone of chemotherapy for epithelial ovarian cancer, however, drug resistance to these agents continues to present challenges. Despite extensive research, the mechanisms underlying this resistance remain unclear.

Materials And Methods: A 2D-gel proteomics method was used to analyze protein expression levels of three human ovarian cancer cell lines and five biopsy samples.

Glycomimetic affinity-enrichment proteomics identifies partners for a clinically-utilized iminosugar.

Widescale evaluation of interacting partners for carbohydrates is an underexploited area. Probing of the 'glyco-interactome' has particular relevance given the lack of direct genetic control of glycoconjugate biosynthesis. Here we design, create and utilize a natural product-derived glycomimetic iminosugar probe in a Glycomimetic Affinity-enrichment Proteomics (glyco-AP) strategy to elucidate key interactions directly from mammalian tissue.

Functional characterization of heat shock protein 90 targeted compounds.

Heat shock protein 90 (Hsp90) is an attractive cancer target that possesses two potential binding domains at the C and N termini. Nevertheless, assays that can reliably distinguish between the C- and N-terminal Hsp90 inhibitors and quantitatively characterize candidate drugs are limited. Here we report an Hsp90 binding assay and ATPase inhibition assay that can not only separate the C- and N-terminal inhibitors but also quantitatively determine their respective IC50 values.