Publications by authors named "Iryna Okhai"
Upregulation of ATP-Sensitive Potassium Channels as the Potential Mechanism of Cardioprotection and Vasorelaxation Under the Action of Pyridoxal-5-Phosphate in Old Rats.
J Cardiovasc Pharmacol Ther
November 2023
Article Synopsis
- The study examines how aging affects protective mechanisms in the body, particularly focusing on ATP-sensitive potassium channels and their role in heart protection via pyridoxal-5-phosphate (PLP) treatment.
- Experiments were conducted on adult and aged rats to assess the impact of PLP on heart health, including mRNA and protein expressions of potassium channels, heart tissue morphology, and responses in cardiac function during stress tests.
- Results indicated that PLP treatment in older rats reduced heart fibrosis, improved heart function under stress, and increased the expression of specific potassium channel subunits while also reducing oxidative stress markers, suggesting a potential therapeutic benefit of PLP through enhanced potassium channel activity and production of protective substances.
Background: In the present work, we investigated the effect of exogenous glutathione in old rats on the expression of ATP-sensitive potassium (K) channels, the mitochondrial permeability transition pore (mPTP) opening in the heart, and the vasorelaxation responses of isolated aortic rings to activation of K channels.
Methods: Experiments were performed on adult (6 months) and old (24 months) male Wistar rats, which were divided into three groups: adult, old, and glutathione-treated old rats. Glutathione was injected intraperitoneally at a dose of 52 mg/kg 1 hour before the studies.
Aging is accompanied by cardiovascular disorders which is associated with an imbalance of pro- and antioxidant systems, the mitochondrial dysfunction, etc. Glutathione (GSH) plays a critical role in protecting cells from oxidative damage. The aim of the work was to study the effect of exogenous glutathione on the redox status of mitochondria, the content of HS and the function of the cardiovascular system in old rats.
View Article and Find Full Text PDFEndothelial Ca-dependent K channels (K) regulate endothelial function. We also know that stimulation of type 2 cannabinoid (CB) receptors ameliorates atherosclerosis. However, whether atherosclerosis is accompanied by altered endothelial K- and CB receptor-dependent signaling is unknown.
View Article and Find Full Text PDFArticle Synopsis
- The study investigated how the endocannabinoid anandamide affects large conductance Ca-dependent K channels in endothelial cells, particularly its receptor-independent effects.
- Anandamide was found to hyperpolarize endothelial cells and increase whole-cell current that was sensitive to specific inhibitors but not affected by G-protein signaling.
- Findings suggest that anandamide directly opens BK channels without needing endothelial cannabinoid receptors, emphasizing its effects on channel activity rather than cell integrity.