A strain of inhibits biofilm formation of caries pathogens via abundant hydrogen peroxide production.

Commensal oral streptococci that colonize supragingival biofilms deploy mechanisms to combat competitors within their niche. Here, we determined that more effectively inhibited biofilm formation of compared to other oral streptococci. This phenotype was common among all isolates of , but was specific to a single strain of , ATCC 49456.

Design, Development and Validation of New Fluorescent Strains for Studying Oral Streptococci.

Bacterial strains that are genetically engineered to constitutively produce fluorescent proteins have aided our study of bacterial physiology, biofilm formation, and interspecies interactions. Here, we report on the construction and utilization of new strains that produce the blue fluorescent protein mTagBFP2, the green fluorescent protein sfGFP, and the red fluorescent protein mScarlet-I3 in species , , and . Gene fragments, developed to contain the constitutive promoter P, the fluorescent gene of interest as well as , providing resistance to the antibiotic spectinomycin, were inserted into selected open reading frames on the chromosome that were both transcriptionally silent and whose loss caused no measurable changes in fitness.

A Strain of Inhibits Biofilm Formation of Caries Pathogens via Abundant Hydrogen Peroxide Production.

Commensal oral streptococci that colonize supragingival biofilms deploy mechanisms to combat competitors within their niche. Here, we determined that more effectively inhibited biofilm formation of within a seven species panel. This phenotype was common amongst all assayed isolates of , but was specific to a single strain of , ATCC 49456.

Human saliva modifies growth, biofilm architecture, and competitive behaviors of oral streptococci.

Unlabelled: The bacteria within supragingival biofilms participate in complex exchanges with other microbes inhabiting the same niche. One example is the mutans group streptococci (s), implicated in the development of tooth decay, and other health-associated commensal streptococci species. Previously, our group transcriptomically characterized intermicrobial interactions between and several species of oral bacteria.

Human Saliva Modifies Growth, Biofilm Architecture and Competitive Behaviors of Oral Streptococci.

The bacteria within supragingival biofilms participate in complex exchanges with other microbes inhabiting the same niche. One example are the mutans group streptococci (s), implicated in the development of tooth decay, and other health-associated commensal streptococci species. Previously, our group transcriptomically characterized intermicrobial interactions between and several species of oral bacteria.