Publications by authors named "Iris Fung"
Despite advances in precision medicine, the clinical prospects for patients with ovarian and uterine cancers have not substantially improved. Here, we analyzed genome-scale CRISPR-Cas9 loss-of-function screens across 851 human cancer cell lines and found that frequent overexpression of SLC34A2-encoding a phosphate importer-is correlated with sensitivity to loss of the phosphate exporter XPR1, both in vitro and in vivo. In patient-derived tumor samples, we observed frequent PAX8-dependent overexpression of SLC34A2, XPR1 copy number amplifications and XPR1 messenger RNA overexpression.
View Article and Find Full Text PDFAberrant activation of the RAS family of guanosine triphosphatases (GTPases) is prevalent in lung adenocarcinoma, with somatic mutation of occurring in ~30% of tumors. We previously identified somatic mutations and amplifications of the gene encoding RAS family GTPase RIT1 in lung adenocarcinomas. To explore the biological pathways regulated by RIT1 and how they relate to the oncogenic KRAS network, we performed quantitative proteomic, phosphoproteomic, and transcriptomic profiling of isogenic lung epithelial cells in which we ectopically expressed wild-type or cancer-associated variants of RIT1 and KRAS.
View Article and Find Full Text PDFArticle Synopsis
- Scientists are using CRISPR technology to understand how certain lung cancer genes, like RIT1, affect cancer growth and treatment options.
- They discovered that RIT1-mutant lung cancer cells are weak spots for specific treatments, especially those that target the cell division process.
- Their findings suggest new ways to help treat lung cancer by focusing on the RIT1 gene and its interactions with other important cancer pathways.
Article Synopsis
- New therapeutic targets are being identified from CRISPR-based screens in adult cancers, raising questions about their applicability to pediatric cancers, which have fewer mutations.
- Researchers created a pioneering pediatric cancer dependency map that included 13 types of pediatric solid and brain tumors, analyzing 82 cell lines with CRISPR-Cas9 to uncover essential genes for survival.
- The study revealed that despite having fewer mutations, pediatric cancer cells exhibit similar complexity in genetic dependencies as adult cancer cells, suggesting that targeted treatments for childhood cancers will need to be developed separately from existing adult oncology drugs.
In the present study, the tetracycline-off and Cre/ systems were combined to gain temporal and spatial control of transgene expression. Mice were generated that carried three transgenes: Tie2-tTA, tet-O-Cre and either the ZEG or ZAP reporter. Tie2-tTA directs expression of tetracycline-controlled transactivator (tTA) in endothelial and hematopoietic cells under the control of the Tie2 promoter.
View Article and Find Full Text PDFThe Notch1 signaling pathway is essential for hematopoietic development. However, the effects of postnatal activation of Notch1 signaling on hematopoietic system is not yet fully understood. We previously generated ZEG‑IC‑Notch1 transgenic mice that have a floxed β‑geo/stop signal between a CMV promoter and intracellular domain of Notch1 (IC‑Notch1).
View Article and Find Full Text PDFObjective: To gain a better understanding of ethnic Chinese women's perceptions and experiences of using barrier and rhythm methods of contraception in order to improve contraceptive counseling at abortion clinics.
Design: Qualitative descriptive study.
Setting: Urban abortion clinic.