Interaction of Liposomes Containing the Carrageenan/Echinochrome Complex with Human HaCaT Keratinocytes In Vitro.

Liposomal drug delivery systems are successfully used in various fields of medicine for external and systemic applications. Marine organisms contain biologically active substances that have a unique structure and exhibit a wide range of biological activities. Polysaccharide of red seaweed (carrageenan (CRG)), and water-insoluble sea urchin pigment (echinochrome (Ech)) interact with each other and form a stable complex.

Carrageenan films as promising mucoadhesive ocular drug delivery systems.

Polymer mucoadhesive films being developed for use in ophthalmology represent a new tool for drug delivery and are considered an alternative to traditional dosage forms. Due to their mucoadhesive properties, carrageenans (CRGs) are widely used in various forms for drug delivery. In this study, films based on CRGs of various structural types (κ/β, κ, x, and λ) for use in ophthalmology were studied.

Carrageenans and Their Oligosaccharides from Red Seaweeds and (Phyllophoraceae) and Their Antiproliferative Activity.

Comparative structural analysis of gelling polysaccharides from and belonging to Phyllophoraceae and the effect of their structural features and molecular weight on human colon cancer cell lines (HT-29, DLD-1, HCT-116) was carried out. According to chemical analysis, IR and NMR spectroscopies, produces kappa/iota-carrageenan with a predominance of kappa units and minor amounts of mu and/or nu units, while the polysaccharide from is iota/kappa-carrageenan (predominance of iota units) and contains negligible amounts of beta- and nu-carrageenans. Iota/kappa- (Afg-OS) and kappa/iota-oligosaccharides (Mp-OS) were obtained from the original polysaccharides through mild acid hydrolysis.

Physicochemical Properties and Antiherpetic Activity of κ-Carrageenan Complex with Chitosan.

Nanoparticles formation is one of the ways to modulate the physicochemical properties and enhance the activity of original polysaccharides. For this purpose, based on the polysaccharide of red algae, κ-carrageenan (κ-CRG), it polyelectrolyte complex (PEC), with chitosan, were obtained. The complex formation was confirmed by ultracentrifugation in a Percoll gradient, with dynamic light scattering.

Antiherpetic Activity of Carrageenan Complex with Echinochrome A and Its Liposomal Form.

Herpes simplex virus (HSV) infections, the incidence of which is still widespread throughout the world, are actualizing the search and development of new, more effective antiherpetic drugs. The development of multifunctional drug delivery systems, including liposome-based ones, has become a relevant and attractive concept in nanotechnology. The ability of complexes of κ- and Σ-carrageenans (CRGs)-sulfated polysaccharides of red algae, with echinochrome A (Ech), as well as the liposomal form of the Σ-CRG/Ech complex-to inhibit different stages of HSV-1 infection in Vero cells was studied.

The Anti-Inflammatory Effect of Carrageenan/Echinochrom Complex at Experimental Endotoxemia.

The anti-inflammatory effects of the CRG/Ech complex in LPS-induced endotoxemia were investigated in vivo in mice and in vitro in LPS-stimulated RAW 264.7 cells and peritoneal macrophages. The results indicated that the CRG/Ech complex suppressed the LPS-induced inflammatory response by reducing the production of ROS and NO in the macrophages.

Mucoadhesive Marine Polysaccharides.

Mucoadhesive polymers are of growing interest in the field of drug delivery due to their ability to interact with the body's mucosa and increase the effectiveness of the drug. Excellent mucoadhesive performance is typically observed for polymers possessing charged groups or non-ionic functional groups capable of forming hydrogen bonds and electrostatic interactions with mucosal surfaces. Among mucoadhesive polymers, marine carbohydrate biopolymers have been attracting attention due to their biocompatibility and biodegradability, sample functional groups, strong water absorption and favorable physiochemical properties.

Influence of the Structural Features of Carrageenans from Red Algae of the Far Eastern Seas on Their Antiviral Properties.

The structural diversity and unique physicochemical properties of sulphated polysaccharides of red algae carrageenans (CRGs), to a great extent, determine the wide range of their antiviral properties. This work aimed to compare the antiviral activities of different structural types of CRGs: against herpes simplex virus type 1 (HSV-1) and enterovirus (ECHO-1). We found that CRGs significantly increased the resistance of Vero cells to virus infection (preventive effect), directly affected virus particles (virucidal effect), inhibited the attachment and penetration of virus to cells, and were more effective against HSV-1.

Comparative Analysis of the Functional Properties of Films Based on Carrageenans, Chitosan, and Their Polyelectrolyte Complexes.

The influence of the structural features of carrageenan on the functional properties of the films was studied. The carrageenans and chitosan films, as well as three-layer films containing a polyelectrolyte complex (PEC) of the two, were prepared. The X-ray diffractograms of carrageenan films reflected its amorphous structure, whereas chitosan and three-layer films were characterized by strong reflection in the regions of 20° and 15° angles, respectively.

Carrageenan gel beads for echinochrome inclusion: Influence of structural features of carrageenan.

Carrageenan (CRG) and carrageenan/chitosan (CH) gel beads (CRG/CH) were prepared as a release delivery system for echinochrome A (Ech). According to spectral data, the Ech was dispersed in the polymer matrix, interacted with CRG, was not oxidised, and remained stable after encapsulation in CRG beads. Carrageenan beads containing Ech were coated with CH by layering.

Current Trend of Marine Carbohydrate-Containing Compounds with Medicinal Properties.

Carbohydrates are most abundant biomolecules on Earth and, also, the most complex biomolecules in terms of structure [...

The Comparative Immunotropic Activity of Carrageenan, Chitosan and Their Complexes.

The immunotropic activity of polyelectrolyte complexes (PEC) of κ-carrageenan (κ-CGN) and chitosan (CH) of various compositions was assessed in comparison with the initial polysaccharides in comparable doses. For this, two soluble forms of PEC, with an excess of CH (CH:CGN mass ratios of 10:1) and with an excess of CGN (CH: CGN mass ratios of 1:10) were prepared. The ability of PEC to scavenge NO depended on the content of the κ-CGN in the PEC.

Inhibitory Effects of Carrageenans on Endotoxin-Induced Inflammation.

The inhibitory effects of carrageenans (CRGs) on lipopolysaccharide (LPS) induced inflammation in a mouse model of endotoxemia and in complex therapy of patients with enteric infections of Salmonella etiology were studied. The atomic force microscopy (AFM) examination of LPS and its mixture with CRGs showed that the LPS morphology is significantly changed under the action of κ- and κ/β-CRGs. CRGs were able to increase the synthesis of anti-inflammatory interleukin 10 (IL-10) in vitro, and, at low concentrations, their activity in the mixture with LPS was higher.

Structural characteristics of carrageenans of red alga Mastocarpus pacificus from sea of Japan.

The sulfated polysaccharide from sterile alga Mastocarpus pacificus was investigated. Partial reductive hydrolysis and NMR spectroscopy showed that the extracted polysaccharides were only carrageenans. According to FT-IR- and NMR spectroscopy this polysaccharide was a hybrid kappa/iota-carrageenan with a predominance of kappa-type units.

Mucoadhesive properties of sulphated polysaccharides carrageenans from red seaweed families Gigartinaceae and Tichocarpaceae.

The mucoadhesive properties of different types of carrageenan (kappa-, kappa/beta-, iota/kappa- and lambda-CRGs) isolated from red seaweed families Gigartinaceae and Tichocarpaceae collected on the Pacific coast were studied. We examined the interaction between CRGs and pig stomach mucin in dilute aqueous solutions using a set of methods. Measurements of the dynamic light scattering of mucin in the presence of CRG showed that the polysaccharides cause aggregation of mucin particles, as confirmed by microscopy data.

Effects of Carrageenans on Biological Properties of Echinochrome.

Sea urchin pigment echinochrome A (Ech), a water-insoluble compound, is the active substance in the cardioprotective and antioxidant drug Histochrome (PIBOC FEB RAS, Moscow, Russia). It has been established that Ech dissolves in aqueous solutions of carrageenans (CRGs). Herein, we describe the effects of different types of CRGs on some properties of Ech.

Liposomal Form of the Echinochrome-Carrageenan Complex.

Inclusion of drugs in liposomes offers the potential for localized and sustained delivery to mucosal surfaces. The inclusion of the carrageenan matrix with echinochrome A ((Ech)-the active substance of the drug Histochrome) in liposomes was studied. According to the spectral characteristics, Ech was not oxidized and retained stability after encapsulation in the liposomes and the lyophilization process.

Carrageenans-Sulfated Polysaccharides from Red Seaweeds as Matrices for the Inclusion of Echinochrome.

The possibility of using different types of carrageenans (CRG) as matrixes for incorporating of echinochrome A (Ech) was investigated. Ech interacts with carrageenans and is incorporated into the macromolecular structure of the polysaccharide. The inclusion of Ech in carrageenan matrices decreased its oxidative degradation and improved its solubility.

Structural analysis and cytokine-induced activity of gelling sulfated polysaccharide from the cystocarpic plants of Ahnfeltiopsis flabelliformis.

Gelling sulfated polysaccharide from the cystocarpic plants of Ahnfeltiopsis flabelliformis was studied. According to FT-IR and NMR spectroscopy data, the polysaccharide was found to be iota/kappa-carrageenan with iota- and kappa-type units in a 2:1 ratio containing beta-carrageenan units and minor amounts of nu- and mu-carrageenans. The HPLC and ESI MS/MS data of enzymatic hydrolysis products revealed that the main components of the polymer chain are iota-carrabiose, iota-carratetraose and hybrid tetra- and hexasaccharides consisting of kappa- and iota-units.

Gelling polysaccharide from Chondrus armatus and its oligosaccharides: the structural peculiarities and anti-inflammatory activity.

Structural peculiarities of low molecular weight (LMW) sample obtained by mild acid hydrolysis of κ-carrageenan from Chondrus armatus was investigated by a rapid mass spectrometric method. The selected conditions allowed avoiding excess destruction of 3,6-AnGal residues that was shown by using tandem (MS/MS) mode. Main oligosaccharide fraction with molecular weight 2.

Structural peculiarities of polysaccharide from sterile form of Far Eastern red alga Ahnfeltiopsis flabelliformis.

KCl-insoluble sulfated polysaccharide from sterile alga Ahnfeltiopsis flabelliformis was investigated. Partial reductive hydrolysis and NMR spectroscopy showed that the polysaccharide comprises disaccharide units of carrabiose only. According to FT-IR-, 1D, 2D NMR spectroscopies and mass-spectrometry this polysaccharide is kappa/beta-carrageenan with ratio of kappa- and beta-types units 3:1 and contains minor amounts of iota- and gamma-carrageenans (precursor of beta-carrageenan).

The properties of chitosan complexes with smooth and rough forms of lipopolysaccharides on CHO-K1 cells.

The negative charge of LPS molecule and the presence of fatty acids in lipid A structure make it capable of binding with chitosan. In the presented work we analyzed the interactions of chitosan with LPS of Burkholderia cepacia or Proteus mirabilis and biological effects of these complexes on CHO-K1 cells. We observed that the presence of O-polysaccharide part of LPS (S1959), core region (R110) or lack of fatty acids in lipid A increased binding affinity of endotoxin with chitosan.

Influence of red algal sulfated polysaccharides on blood coagulation and platelets activation in vitro.

The influence of sulfated polysaccharides (λ-, κ-, and κ/β-carrageenan and porphyran) - on platelet activation was studied. Carrageenans were much weaker inhibitors of a coagulation process than heparin, while porphyran had not that effect. Results of the aPTT and PT assays suppose that carrageenans affected mostly intrinsic pathway of coagulation, while their effect on the extrinsic pathway is extremely low (λ and κ/β) or absent (κ, LMW derivative of κ-carrageenan).

Effects of structural peculiarities of carrageenans on their immunomodulatory and anticoagulant activities.

Biological activity of five carrageenan types - kappa, kappa/beta, kappa/iota, lambda and new type - iks - isolated from the most abundant species belonging to Gigartinaceae and Tichocarpaceae collected from the Pacific coast was investigated. The ability of carrageenans to influence on the cytokine production by human cells is greatly dependent on concentration and structure of polysaccharides. At high concentrations all types of carrageenans increased the level of pro-inflammatory IL-6 and TNF-α, while at low concentration (1-10ng/mL) their activity was insignificant.