The Native Monomer of Bacillus Pumilus Ribonuclease Does Not Exist Extracellularly.

Supported by crystallography studies, secreted ribonuclease of (binase) has long been considered to be monomeric in form. Recent evidence obtained using native polyacrylamide gel electrophoresis and size-exclusion chromatography suggests that binase is in fact dimeric. To eliminate ambiguity and contradictions in the data we have measured conformational changes, hypochromic effect, and hydrodynamic radius of binase.