Publications by authors named "Irina Krier"
Huntington's disease is a severe but slowly progressive hereditary illness for which only symptomatic treatments are presently available. Clinical measures of disease progression are somewhat subjective and may require years to detect significant change. There is a clear need to identify more sensitive, objective and consistent measures to detect disease progression in Huntington's disease clinical trials.
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- Many organisms, including mammals, display daily patterns in gene expression that affect physiological processes, with these rhythms influenced by circadian clocks and feeding cycles.
- In a study of mouse liver, researchers identified dynamic DNase I hypersensitive sites (DHSs) that indicate where regulatory elements are located, showing that these sites cycle in coordination with the activity of RNA polymerase II and specific histone modifications.
- The findings suggest that, although rhythmic gene expression persists without the core clock protein Bmal1, its absence leads to reduced amplitude in these rhythms due to the influence of transcriptional regulators connected to feeding and systemic signals.
The cellular abundance of transcription factors (TFs) is an important determinant of their regulatory activities. Deriving TF copy numbers is therefore crucial to understanding how these proteins control gene expression. We describe a sensitive selected reaction monitoring-based mass spectrometry assay that allowed us to determine the copy numbers of up to ten proteins simultaneously.
View Article and Find Full Text PDFThe molecular role of corepressors is poorly understood. Here, we studied the transcriptional function of the corepressor SMRT during terminal adipogenesis. Genome-wide DNA-binding profiling revealed that this corepressor is predominantly located in active chromatin regions and that most distal SMRT binding events are lost after differentiation induction.
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