Assessment of metabolic activity of human cells in solution and in polymer matrix with the use of metabolite-sensitive sensors.

We developed metabolite-sensitive electrochemical sensors on the basis of electrodes modified with a thick film of carbon nanotubes. Modified electrodes provide efficient pre-adsorption of cellular metabolites and their sensitive detection using anodic square-wave voltammetry. On the electrode surface both adhered and non-adhered human cells produce three oxidation peaks at the potentials of +0.

Electrochemical sensor for blood deoxyribonucleases: design and application to the diagnosis of autoimmune thyroiditis.

We designed an electrochemical sensor based on a carbon nanotube modified electrode (ME) to analyze DNA-cleaving activity. The cleavage of high molecular weight DNA resulted in an increase in the oxidation current from DNA guanine nucleotides due to a change in DNA adsorptive behavior on the surface of the ME. DNA digestion with DNAse I was accompanied by a linear increase in the DNA signal in proportion to the enzyme activity.

Binding and purification of plasmid DNA using multi-layered carbon nanotubes.

We propose a new method for the separation of nucleic acids using multi-layered carbon nanotubes (CNTs) as an adsorbent. According to agarose gel electrophoresis, oxidized water-stable CNTs adsorb certain forms of nucleic acids, such as high molecular weight RNA, chromosomal DNA, linear and denatured forms of plasmid DNA. However, CNTs do not adsorb supercoiled form of plasmid DNA.