A lubricious formulation exhibiting reduced thrombogenicity, cell proliferation, and protein adsorption.

The adhesion of human platelets, erythrocytes, and leukocytes, the adsorption of protein, and the proliferation of human umbilical vein endothelial cells (HUVEC) on the surface of electropolished stainless steel and the lumen of polyurethane tubing coated with Hydromer's lubricious Duality T8B formulation was evaluated. Following exposure to a platelet-enriched suspension from citrated human whole blood, stainless steel coated with this formulation exhibited significantly reduced adhesion of platelets, erythrocytes, and granulocytes. This reduction in adhesion was confirmed using an immunohistochemical method utilizing antibodies to CD41, CD235, and CD15, respectively.

Cell adhesion and proliferation are reduced on stainless steel coated with polysaccharide-based polymeric formulations.

Hydromer's polymeric formulations F200 and F202 were evaluated after application to a synthetic substrate for effects on cell adhesion and proliferation. A significant reduction in cell adhesion was observed when cells grown on medical-grade stainless steel coated with these polymers were stained and examined under a fluorescence microscope. This reduction in cell adhesion/proliferation was confirmed when cells were isolated and analyzed by the MTS cell proliferation assay.