Publications by authors named "Iria Bravo"
Carbon nanodots modified with Neutral Red covalently inserted in the nanostructure (NR-CDs) have been prepared by a simple synthesis method based on microwave irradiation under controlled temperature and pressure. The synthetized NR-CDs have been characterized by different techniques, demonstrating the covalent bonding of Neutral Red molecules to the carbon dots nanostructure. Fluorescence activity of the prepare NR-CDs has been explored showing different interaction pathways with singled and doubled stranded DNA.
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- BPA is an endocrine disruptor found in aquatic ecosystems, prompting the creation of an electrochemical biosensor using the enzyme laccase from Trametes versicolor.
- The biosensor was optimized with a bioconjugate that combines laccase, chitosan, and an ionic liquid, alongside being enhanced by multi-walled carbon nanotubes for better performance.
- It demonstrated impressive sensitivity, a low detection limit, and maintained accuracy and stability when analyzing BPA levels in river water, showcasing its effectiveness for environmental monitoring.
This work reports on the advantages of using carbon nanodots (CNDs) in the development of reagent-less oxidoreductase-based biosensors. Biosensor responses are based on the detection of HO, generated in the enzymatic reaction, at 0.4 V.
View Article and Find Full Text PDFThe authors report on a fluorometric method for the rapid detection of BRCA1, CFRT and MRP3 gene mutations. These are associated with breast cancer, cystic fibrosis and autoimmune hepatitis diseases, respectively. Carbon nanodots with blue fluorescence (with excitation/emission maxima at 340/440 nm) were synthesized and characterized, and their interactions with DNA were investigated.
View Article and Find Full Text PDFNew methods for the production of colloidal Ga nanoparticles (GaNPs) are introduced based on the evaporation of gallium on expendable aluminum zinc oxide (AZO) layer. The nanoparticles can be prepared in aqueous or organic solvents such as tetrahydrofuran in order to be used in different sensing applications. The particles had a quasi mono-modal distribution with diameters ranging from 10 nm to 80 nm, and their aggregation status depended on the solvent nature.
View Article and Find Full Text PDFLactic acid is a relevant analyte in the food industry, since it affects the flavor, freshness, and storage quality of several products, such as milk and dairy products, juices, or wines. It is the product of lactose or malo-lactic fermentation. In this work, we developed a lactate biosensor based on the immobilization of lactate oxidase (LOx) onto ,'-Bis(3,4-dihydroxybenzylidene) -1,2-diaminobenzene Schiff base tetradentate ligand-modified gold nanoparticles (3,4DHS-AuNPs) deposited onto screen-printed carbon electrodes, which exhibit a potent electrocatalytic effect towards hydrogen peroxide oxidation/reduction.
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- The study introduces 3,4-dihydroxysalophen Schiff base (3,4-DHS) as a multifunctional ligand for synthesizing gold nanoparticles (AuNPs) with unique properties.
- 3,4-DHS can effectively reduce gold chloride (HAuCl4) in water and also functions as a stabilizing agent, leading to stable colloidal AuNPs with controlled sizes in a single reaction process.
- When applied to carbon electrodes, the 3,4-DHS-AuNPs demonstrate significant electrocatalytic activity for the oxidation of hydrazine and for the oxidation/reduction of hydrogen peroxide.