Simple and Rapid Site-Specific Integration of Multiple Heterologous DNAs into the Escherichia coli Chromosome.

Escherichia coli is the most studied and well understood microorganism, but research in this system can still be limited by available genetic tools, including the ability to rapidly integrate multiple DNA constructs efficiently into the chromosome. Site-specific, large serine-recombinases can be useful tools, catalyzing a single, unidirectional recombination event between 2 specific DNA sequences, and without requiring host proteins for functionality. Using these recombinases, we have developed a system to integrate up to 12 genetic constructs sequentially and stably into in the E.

Methylome and Complete Genome Sequence of Parageobacillus toebii DSM 14590, a Thermophilic Bacterium.

Here, we present the first complete genome assembly of the thermophilic bacterium DSM 14590 The DSM 14590 genome consists of a 3,270,071-bp circular chromosome and a 52,989-bp native plasmid.