Trivalent immunization with metal-binding proteins confers protection against enterococci in a mouse infection model.

is ranked among the top five bacterial pathogens responsible for catheter-associated urinary tract infections, wound infections, secondary root canal infections, and infective endocarditis. Previously, we showed that inactivation of either the manganese- and iron-binding (EfaA) or zinc-binding (AdcA and AdcAII) lipoproteins significantly reduced virulence. Here, we explored whether immunization using a multi-valent approach induces protective immunity against systemic enterococcal infections.