Publications by authors named "Irene R A M Ogutu"
Article Synopsis
- The metal binding motif in nitrile hydratases (NHases) is conserved and characterized by the sequence CXXCSCX in the α-subunit, with a synthesized peptide (VCTLCSCY) shown to coordinate Fe(II) under anaerobic conditions.
- EPR analysis confirmed the presence of an integer-spin signal in the Fe(II)-peptide complex, suggesting it has a distinct electronic structure with small axial zero-field splitting (ZFS).
- Exposure to air leads to changes in the EPR signals, indicating a transition from high-spin to low-spin Fe forms, revealing a novel progression of iron oxidation in NHases that includes both high-spin and low-spin states.
