Development and Characterization of a Porcine Liver Scaffold.

The growing number of patients requiring liver transplantation for chronic liver disease cannot be currently met due to a shortage in donor tissue. As such, alternative tissue engineering approaches combining the use of acellular biological scaffolds and different cell populations (hepatic or progenitor) are being explored to augment the demand for functional organs. Our goal was to produce a clinically relevant sized scaffold from a sustainable source within 24 h, while preserving the extracellular matrix (ECM) to facilitate cell repopulation at a later stage.

Creation of an Acellular Vaginal Matrix for Potential Vaginal Augmentation and Cloacal Repair.

Study Objective: Our aim was to use porcine vagina to create a vaginal matrix and test its cellular biocompatibility.

Design, Setting, And Participants: Vagina was harvested from pigs and decellularized (DC) using a combination of detergents (Triton X-100 and sodium deoxycholate) and enzymes (DNAse/RNAse).

Interventions: The presence of cellular material, collagen structural integrity, and basement membrane proteins were assessed histologically.