The substratum preferences of spined loach from eastern England differed significantly between fish of different age groups, sexes, habitat origin (river or drainage ditches) and even between ditches, under experimental conditions. Spined loach from drainage ditches preferred organic sediments, while those from a river showed more variable substratum choice. Significant inter-individual differences were found in river fish, with juveniles preferring sand, while males preferred organic sediment and females gravel.
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