Transcriptomic analysis reveals differential gene expression patterns of Lacticaseibacillus casei ATCC 393 in response to ultrasound stress.

In recent years, there has been a growing interest in modulating the performance of probiotic, mainly Lactic Acid Bacteria (LAB), in the field of probiotic food. Attenuation, induced by sub-lethal stresses, delays the probiotic metabolism, and induces a metabolic shift as survival strategy. In this paper, RNA sequencing was used to uncover the transcriptional regulation in Lacticaseibacillus casei ATCC 393 after ultrasound-induced attenuation.

Microencapsulation and sonication: A multiple physical approach to attenuate the probiotic ATCC 393.

Modulation of probiotic performances represents a tool to avoid the probiotic off-flavor in probiotic food. Microencapsulation and sonication were evaluated in slowing down the ATCC 393 induced acidification. Firstly, the influence of alginate concentration and chitosan coating on acidification rate were tested.

Ultrasound Attenuation Improves Some Surface Properties of the Probiotic Strain ATCC 393.

Ultrasound attenuation has been recently proposed as a tool to modulate probiotic metabolism. The study aimed to characterize the response of the probiotic Lacticaseibacillus casei ATCC 393 to sonication. Two ultrasound treatments were tested (57 W, duty cycle 50%, 6 or 8 min).

Application of ultrasound and microencapsulation on DSM 17938 as a metabolic attenuation strategy for tomato juice probiotication.

Counteracting probiotic-induced physicochemical and sensory changes is a challenge in the development of probiotic beverages. The aim of the study is to apply ultrasound and microencapsulation for the attenuation of DSM 17938 to avoid change in a probiotic tomato juice. Preliminarily, six ultrasound treatments were applied.

Effect of Microencapsulation on Survival at Simulated Gastrointestinal Conditions and Heat Treatment of a Non Probiotic Strain, 48M, and the Probiotic Strain DSM 17938.

Cells of the probiotic strain DSM 17938 and of the non-probiotic strain 48M were microencapsulated in alginate matrix by emulsion technique. Survival of microorganisms in the microcapsules was tested against gastrointestinal (GI) simulated conditions and heat stress. Results demonstrated that the microencapsulation process improved vitality of 48M cells after GI conditions exposure, allowing survival similarly to the probiotic DSM 17938.