Large-scale preparation of sodium-potassium ATPase from kidney outer medulla.

Background: Large amounts of Na,K-ATPase are needed for studies involving protein chemistry. Preparation of Na,K-ATPase from kidney by the widely used, rapid procedure of Jørgensen (Biochim Biophys Acta 356:36-52, 1974; Methods Enzymol 156:29-43, 1988) includes labor-intensive dissection of tissue from the outer medulla and centrifugation into a step gradient of sucrose solution.

Methods: In a large-scale modification presented here, tissue was dissected with a surgical instrument, a rongeur, and centrifugation was simply a five times repeated differential centrifugation.