Complete genome sequence of Vibrio diabolicus bacteriophage vB_Vc_SrVc2 and its efficacy as prophylactic phage therapy.

Vibrio diabolicus is widely distributed in the marine environment and is an important pathogen of aquatic organisms such as shrimp, fish, and mollusks. The emergence of multi-drug resistance among these bacteria has resulted in a global public health problem, which requires alternative treatment approaches, such as phage therapy. In the present study, we isolated the phage vB_Vc_SrVc2 from the hepatopancreas of white shrimp showing symptoms of acute hepatopancreatic necrosis disease (AHPND) and evaluated the efficacy of this phage in preventing the mortality associated with V.

Transcriptional analysis in bacteriophage Fc02 of revealed two overlapping genes with exclusion activity.

Little is known about the gene expression program during the transition from lysogenic to lytic cycles of temperate bacteriophages in . To investigate this issue, we developed a thermo-sensitive repressor mutant in a lysogen and analyzed the phage transcriptional program by strand-specific RNA-Seq before and after thermo-induction. As expected, the repressor gene located on the phage DNA forward strand is transcribed in the lysogen at the permissive temperature of 30°C.