Fluorescence detection of the ornamental fish Cardinal Tetra (Paracheirodon axelrodi).

The fluorescence spectra of the tropical fish, Cardinal Tetra (Paracheirodon axelrodi), originating in the Amazon region of Brazil, were determined. These spectra were then treated using factor analysis, generating two contributing spectra and separating out the noise. Time-resolved fluorescence results indicated that the fluorescent system in the epidermis undergoes excited state reaction.

The complex between 9-(n-decanyl)acridone and bovine serum albumin. Part 2. What do fluorescence probes probe?

Factor analysis indicates that the fluorescence spectrum of 9-(n-decanyl)acridone (NDA), when bound to Bovine Serum Albumin (BSA), can be described quite adequately as the sum of two spectra, attributed to a "free" and a "bound" species. Kinetic evidence indicates that upon electronic excitation the system undergoes a net increase in free NDA, relative to the equilibrium distribution in the ground state, which would be consistent with Lewis acid sites on BSA being responsible for the binding. The system does not attain a position of equilibrium during the duration of the excited singlet state.