Further evidence of biallelic NAV3 variants associated with recessive neurodevelopmental disorder with dysmorphism, developmental delay, intellectual disability, and behavioral abnormalities.

Neuron navigators (NAVs) are cytoskeleton-associated proteins well known for their role in axonal guidance, neuronal migration, and neurite growth necessary for neurodevelopment. Neuron navigator 3 (NAV3) is one of the three NAV proteins highly expressed in the embryonic and adult brain. However, the role of the NAV3 gene in human disease is not well-studied.

Development of Timolol Maleate-Loaded Poloxamer-co-Poly (acrylic acid) based hydrogel for controlled drug delivery.

Free radical polymerization technique was used to formulate Poloxamer-188 based hydrogels for controlled delivery. A total of seven formulations were formulated with varying concentrations of polymer, monomer ad cross linker. In order to assess the structural properties of the formulated hydrogels, Fourier Transform Infrared Spectroscopy (FTIR), Thermogravimetric analysis (TGA), Differential Scanning Calorimetry (DSC), Scanning electron microscopy (SEM), and X-ray diffraction (XRD) were carried out.

An In-Depth Insight into Clinical, Cellular and Molecular Factors in COVID19-Associated Cardiovascular Ailments for Identifying Novel Disease Biomarkers, Drug Targets and Clinical Management Strategies.

COVID-19 was initially identified as a respiratory system disorder, but it has been reported to interact with and influence the cardiovascular system, in addition to many other body systems. Although COVID-19-associated cardiovascular (CV) complications are common, resulting in high acute phase mortality and a large number of morbidities in the chronic phase, thus severely impacting patients' quality of life and health outcomes, yet clinical, cellular, and molecular biological factors underlying the pathophysiology of cardiovascular complications associated with COVID-19 are poorly understood. This review investigates putative underlying clinical factors as well as cellular and molecular biological mechanisms by which COVID-19 leads to acute CV complications, including state-of-the-art genomic sequencing-based findings, and assessing the long-term CV consequences of COVID-19, aiming to shed light on developing strategies for differential diagnosis, risk prognostic stratification, prevention, and clinical management of CV sequels in COVID-19 patients.

Impact of fresh grape juice on the pharmacokinetics of omeprazole: results of a food-drug interaction study.

Grapes have been widely used for dietary ailments due to their attributed pharmacological activities. Resveratrol, the chief constituent of grapes, is responsible for their pharmacological benefits. However, apart from their beneficial effects, grapes have also recently been considered in drug interaction studies.

Metabolic-Engineering Approach to Enhance Vanillin and Phenolic Compounds in Ocimum Sanctum (CIM-Angana) via VpVAN Overexpression.

Transgenic Ocimum sanctum plants were engineered to produce vanillin by overexpressing the VpVAN gene using Agrobacterium-mediated transformation. Positive transformants developed shoots within 4-5 weeks and were transferred to a root induction medium and four independent transformants with no observed adverse effects were kept for anlysis. Quantitative RT-PCR indicated significantly higher VpVAN expression in transgenic lines AG_3 and AG_1, impacting the phenylpropanoid pathway and phenolic compound accumulation.

The review of the genus (Coleoptera, Coccinellidae) from Pakistan.

Background: The genus is reviewed with seven species found in Pakistan: (Mulsant, 1866), Kapur, 1973, Dobzhansky, 1926, Fabricius, 1781, Linnaeus, 1758, Faldermann, 1835 and Linnaeus, 1758. Information on prey, host plants, distribution and an identification key for species in Pakistan is provided. Additionally, newly-sequenced partial COI (cytochrome-c-oxidase subunit I) for and were used to determine their phylogenetic positions within the genus .

Computer-aided rational design of a mRNA vaccine against Guanarito mammarenavirus.

Purpose: Guanarito mammarenavirus (GTOV) is a highly pathogenic virus that leads to Venezuelan hemorrhagic fever (VHF). Despite being a severe disease, there are currently no commercially available drugs or vaccines for its prevention.

Methods: Here we computationally formulated a mRNA vaccine construct (VC) from the genome of GTOV to produce immunity against its infections.

Development and Validation of Rapid and Cost-Effective RP-HPLC/UV Method for Determination of Cetirizine/Levocetirizine in Blood Plasma of Smokers and Non Smokers: a Two Cohort Comparative Study.

In this research study, a simple, rapid, sensitive, and cost-effective Reverse Phase High Performance Liquid Chromatography method was developed and validated for determination of Cetirizine and Levocetirizine in human plasma. The drugs were separated on an ACE Generix100-5 C18 RP (250 × 4.6 mm, 5 μm) column, using Acetonitrile and Distilled water pH 7 (59:41 v/v) as mobile phase with a flow rate of 1.

Estimation of genetic polymorphism in quince (Cydonia oblonga Mill.) genotypes using morphological traits and molecular (DNA barcoding) characterizations.

Quince (Cydonia oblonga) is a medicinal plant and a member of family Rosaceae. It is native plant of Asia Minor and Europe. It is used in production of jam and jellies and also as a remedy of several ailments.

Cost-effective Skin Marking in Joint Replacement Surgery: A Comparative Study between Methylene Blue Ink and Commercially Available Skin Markers.

Introduction: This study aimed to assess and compare the effectiveness and safety of methylene blue ink and commercially available surgical marker pens in pre-operative skin marking for joint replacement procedures. The objective was to evaluate the visibility of the surgical instruments during the operation and their influence on the occurrence of infections after the surgery, providing valuable information on the practical use and cost-effectiveness.

Materials And Methods: This retrospective randomized study involved 150 total cases, which included knee replacements, total hip replacements, and hip hemiarthroplasty performed between 2020 and 2023.

Multidisciplinary Innovations in Managing Complex Rheumatic Valve Disease in Low- and Middle-Income Countries: Insights From the NICVD Heart Valve Registry in Pakistan.

The National Institute of Cardiovascular Diseases Heart Valve Registry prospectively enrolled 1218 consecutive patients referred for surgery for valvular heart disease. A multidisciplinary management approach was used changing the plan from surgical to catheter based or medical in a significant number of patients. Rheumatic heart disease was the most common type of valve disease.

Self-assembled latanoprost loaded soluplus nanomicelles as an ophthalmic drug delivery system for the management of glaucoma.

Glaucoma, a leading cause of blindness due to elevated intraocular pressure (IOP), is managed with medications like latanoprost (LAT), a prostaglandin analogue, to enhance aqueous outflow. Despite the challenge posed by eye anatomy and tear dynamics, effective ocular bioavailability via topical administration remains elusive. This study aims to optimize self-assembled nanomicelles incorporating LAT, an anti-glaucoma drug, belonging to BCS Class II (low solubility and high permeability) via a two-level, two-factor full factorial design, the nanomicelles were formulated via direct dissolution method and validated using design of expert.

Assessing the Effectiveness of a Short Injectable Treatment Regimen for Multidrug-Resistant Tuberculosis: Clinical Outcomes in Khyber Pakhtunkhwa.

Background The currently recommended treatment regimens for multidrug-resistant tuberculosis (MDR-TB) are complex, costly, prolonged (lasting at least 20 months), and often poorly tolerated. The objective of this study was to evaluate the effectiveness of the shorter treatment regimen (STR) for treating patients with MDR-TB in Khyber Pakhtunkhwa. Methodology The present study was a case series conducted at the Programmatic Management of Drug Resistant TB Unit, Lady Reading Hospital (PMDT-LRH), Khyber Pakhtunkhwa, Pakistan.

Anti-anemic potential of L. in iron-deficient rat model; network pharmacology profiling.

Iron deficiency anemia is a global health concern, affecting around 2 billion people. Oral iron therapy often causes severe gastro-intestinal issues. , member of the Brassicaceae family, is valued in traditional medicine and renowned for its rich iron and vitamin C content.

Train-of-four ratio, counts and post tetanic counts with the Tetragraph electromyograph in comparison to mechanomyography.

Automated EMG devices to detect compound muscle action potentials from the adductor pollicis muscle in response to ulnar nerve stimulation, regardless of hand and thumb position, may serve as a better reference ("gold standard") for clinical assessment of neuromuscular function than traditional mechanomyography (MMG) systems that need custom design and validation in lab settings. This evaluation compared the TetraGraph EMG system against a validated MMG device to investigate the accuracy and repeatability of this quantitative EMG monitor for detecting onset, offset and deep neuromuscular block. Simultaneous muscle action potential recordings from the EMG neuromuscular monitor and muscle contractions from an in-house developed MMG monitor in response to ulnar nerve stimulation were obtained from patients having elective surgery requiring neuromuscular block.

Association Between Sleeping Quality and Risk of Breast Cancer Among Women: A Case-Control Study From Pakistan.

Background: This case-control study investigated the relationship between sleep duration and quality with the occurrence of breast cancer among women, both pre- and post-menopausal, in the northwest Khyber Pakhtunkhwa (KP) region of Pakistan.

Method: This case-control research was carried in multiple tertiary care facilities. Newly diagnosed primary breast cancer patients were recruited as cases (n = 408), and 5+ years age-matched controls (n = 408) were randomly selected from the general population.

Unlocking the Potential of Origanum Grosii Essential Oils: A Deep Dive into Volatile Compounds, Antioxidant, Antibacterial, and Anti-Enzymatic Properties within Silico Insights.

The present study aimed to comprehensively characterize the volatile compounds from the aerial parts of Origanum grosii and evaluate their potential as antioxidants and enzyme inhibitors through both in vitro and in silico approaches. The essential oil's volatile constituents were identified using Gas Chromatography-Mass Spectrometry (GC-MS) analysis, revealing carvacrol (31 %), p-cymene (18.59 %), thymol (12.

Bioactivity of a dihydroxy flavone produced by EU0013 against the wilt pathogen of tomato.

Bioassay-guided isolation of a dihydroxy flavone from the cell extract of EU0013 was carried out using preparative LCMS and the structure was interpreted using 1D and 2D NMR spectra. The fungus was fermented in three different nutrient media Glucose Nutrient Broth, Glucose Peptone Yeast Broth, and Yeast Extract Broth and the cell extract was obtained using organics. Comparative LCMS profiles of the fungus confirmed the enhanced production of dihydroxy flavone in GPYB media.

Multifaceted natural drought response mechanisms in three elite date palm cultivars uncovered by expressed sequence tags analysis.

This study extends our prior research on drought responses in three date palm cultivars (Khalas, Reziz, and Sheshi) under controlled conditions. Here, we investigated their drought stress adaptive strategies under ambient environment. Under natural field drought conditions, three date palm cultivars experienced significantly (p ≤ 0.

From lab to field: validity and reliability of inertial measurement unit-derived gait parameters during a standardised run.

The aim was to assess concurrent validity and test-retest reliability of spatiotemporal gait parameters from a thoracic-placed inertial measurement unit (IMU) in lab- (Phase One) and field-based (Phase Two) conditions. Spatiotemporal gait parameters were compared (target speeds 3, 5 and 7.5 m·s) between a 100 Hz IMU and an optical measurement system (OptoJump Next, 1000 hz) in 14 trained individuals (Phase One).

Green enrichment of argan oil ( L.) with thyme ( L.) and oregano ( L.) leaves: Evaluating quality and stability improvements.

This study aimed to assess the impact of enriching argan oil (AO) ( L.) using the maceration technique with thyme ( L.) and oregano ( L.