Publications by authors named "Ipsita K Sen"

Antimicrobial wound dressings offer enhanced efficacy compared to conventional dressing platforms by limiting bacterial infections, expediting the healing process, and creating a barrier against additional wound contamination. The use of silk derived from silkworm cocoons in wound healing applications is attributed to its exceptional characteristics. Compared to mulberry silk, sericin from non-mulberry cocoons has higher water exchange mobility and moisture retention.

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Article Synopsis
  • - A water-soluble xylan (PS-I) was extracted from fresh Piper betle leaves, with a molecular weight of around 1.1 x 10^4 Da, and composed mainly of xylose, galactose, and methyl galacturonate in a 3:1:1 ratio.
  • - Its structure features a backbone of three (1 → 4)-α-D-xylopyranosyl residues, one of which branches out with a side chain of (1 → 4)-α-D-galacturonic acid methyl ester and terminal β-D-galactose.
  • - The xylan showed significant immunostimulatory effects on immune cells and demonstrated strong antioxidant properties, suggesting potential uses
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The emergence of the novel coronavirus, SARS-CoV-2 has pushed forward the world to experience the first pandemic of this century. Any specific drug against this RNA virus is yet to be discovered and presently, the COVID-19 infected patients are being treated symptomatically. During the last few decades, a number of polysaccharides with potential biological activities have been invented from Indian medicinal plants.

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Mushrooms are renewable natural gift for humankind, furnished with unique taste, flavor and medicinal properties. For the last few decades study of mushroom polysaccharides has become a matter of great interest to the researchers for their immunomodulating, antimicrobial, antioxidant, anticancer, and antitumor properties. Molecular mass, branching configuration, conformation of polysaccharides and chemical modification are the major factors influencing their biological activities.

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A water soluble polysaccharide (PGPS) with molecular weight ~ 1.4 × 10 Da was isolated by alkali treatment from an edible mushroom Polyporus grammocephalus and purified by gel chromatography using sepharose-6B column. Monosaccharide analysis revealed that PGPS was made up of glucose only.

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A water soluble heteroglycan (THPS) of an average molecular weight ~1.98 × 10 Da was isolated from the aqueous extract of the fruit bodies of an edible mushroom Termitomyces heimii. Structural characterization of THPS was carried out using acid hydrolysis, methylation analysis, periodate oxidation, Smith degradation and 1D/2D NMR studies.

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Herein, we report the synthesis of silver nanoparticles (AgNPs) by a green route using the aqueous leaf extract of Morus indica L. V1. The synthesized AgNPs exhibited maximum UV-Vis absorbance at 460 nm due to surface plasmon resonance.

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A novel exopolysaccharide (EPS) was produced by a bacterium which was isolated from Psophocarpus tetragonolobus (L) D.C. and identified as 99% Rhizobium tropici SRA1 by 16S rDNA sequencing.

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A water-soluble heteroglycan (PS-I) isolated from the aqueous extract of a wild edible mushroom Lentinus sajor-caju showed average molecular weight ∼1.79×10Da. The structure of the polysaccharide was determined using chemical and 1D/2D NMR experiments.

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A new water soluble glucan (MGPS), with a molecular weight ∼1.48×10Da, was isolated from the fruit bodies of Meripilus giganteus by hot water extraction followed by purification through dialysis tubing cellulose membrane and sepharose 6B column chromatography. Its structural characteristics were investigated by acid hydrolysis, methylation analysis, Smith degradation and 1D/2D NMR experiments.

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A water-soluble heteroglycan (PS-II) with average molecular weight ∼7.27×10Da, was isolated from the fruiting bodies of an edible truffle mushroom Tuber rufum (Pico) var. by hot water extraction.

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Silver nanoparticles (AgNPs) were synthesized using a hetero polysaccharide (PS) isolated from Lentinus squarrosulus (Mont.) Singer. The polysaccharide fraction (consisting of glucose, fucose and galactose) serves the role of both reducing as well as stabilizing agent.

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A water-soluble heteroglycan (PS) of an average molecular weight ∼1.98 ×10(5) Da was isolated from the aqueous extract of an edible mushroom Termitomyces clypeatus (R. Heim).

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A water soluble fucogalactan (PS-II) of an average molecular weight ∼1.2×10(5) Da was isolated from the aqueous extract of an edible mushroom Macrolepiota dolichaula. It was composed of fucose, galactose and 3-O-methyl galactose in a molar ratio of nearly 1:4:1.

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An exopolysaccharide (KNPS) of an average molecular weight ∼1.8×10(5) Da was isolated from the culture medium of Klebsiella pneumoniae PB12. Structural characterization of KNPS was carried out using sugar and methylation analysis, Smith degradation and 1D/2D NMR experiments.

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A water soluble β-glucan having molecular weight ∼2×10(5)Da was isolated from hot water extract of the fruit bodies of an edible mushroom Entoloma lividoalbum (Kühner & Romagn) Kubička. This polysaccharide (ELPS) contains (1→3,6)-β-D-Glcp, (1→3)-β-D-Glcp, (1→6)-β-D-Glcp, and terminal β-D-Glcp moieties in a molar ratio of nearly 1:1:3:1. Chemical and spectroscopic analysis showed that the backbone of glucan consists of three (1→6)-β-D-glucopyranosyl and two (1→3)-β-D-glucopyranosyl residues, out of which one (1→3)-β-D-glucopyranosyl moiety was branched at O-6 with a terminal β-D-glucopyranosyl residue.

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A water soluble heteroglycan (PS-II) of an average molecular weight ∼5.2×10(4)Da was isolated from the alkaline extract of an edible mushroom Entoloma lividoalbum (Kühner & Romagn) Kubička. Structural characterization of PS-II was carried out using sugar and methylation analysis, periodate oxidation study, and 1D/2D NMR experiments.

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A new water-soluble heteropolysaccharide (PS-II) with apparent molecular weight ∼1.65×10(5) Da, was isolated from the fruiting bodies of hybrid mushroom pfle 1p by hot aqueous extraction. It is composed of d-mannose, d-galactose, and 3-O-Me-d-galactose in a molar ratio of 1.

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A water-soluble heteropolysaccharide (PS) was isolated from extracellular polymeric substances (EPS) produced by a novel metal tolerant bacterium, Acinetobacter junii BB1A. Sugar analysis showed that the PS was composed of mannose, galactose and arabinose in a molar ratio of nearly 3:1:1. Structural characterization of the PS was carried out using methylation analysis, periodate oxidation, Smith degradation and 1D/2D NMR experiments.

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A water soluble β-glucan (PS) with an average molecular weight ∼1.95 × 10(5)Da was isolated from the alkaline extract of ectomycorrhizal edible mushroom, Russula albonigra (Krombh.) Fr.

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A water soluble branched β-D-glucan (PS-I) with an average molecular weight ~2.1×10(5) Da was isolated from alkaline extract of the fruit bodies of the edible mushroom Entoloma lividoalbum (Kühner & Romagn) Kubička which consists of terminal β-D-glucopyranosyl, (1→3)-β-D-glucopyranosyl, (1→6)-β-D-glucopyranosyl, and (1→3,6)-β-D-glucopyranosyl moieties in a molar ratio of nearly 1:3:2:1. The structure of PS-I was elucidated using acid hydrolysis, methylation analysis, periodate oxidation study, partial hydrolysis, and 1D/2D NMR experiments.

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This work demonstrates synthesis of silver nanoparticles (AgNPs) using glucan isolated from a mushroom Pleurotus florida blue variant. UV-vis spectroscopy showed maximum absorbance at 425 nm due to surface plasmon resonance of AgNPs. Average diameter of the synthesized AgNPs was 2.

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A water soluble branched glucan (PS-I) was isolated from aqueous extract of the fruit bodies of an edible mushroom Macrolepiota dolichaula, having average molecular weight ~2.02×10(5) Da. The structure of this PS-I was determined using total hydrolysis, methylation analysis, Smith degradation, partial hydrolysis, and 1D/2D NMR experiments.

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A water-soluble heteropolysaccharide (PS-I) having molecular weight ~2.1×10(5) Da was isolated from hot aqueous extract of the fruit bodies of hybrid mushroom pfle 1p. The hybrid mushroom pfle 1p was obtained through intergenic protoplast fusion between Pleurotus florida and Lentinula edodes.

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