[Distinctions in molecular composition of the dental biofilm in a dependence of method of exo/endogeneous caries prevention and cariogenic condition of a patient].

Objective: Comparative study of changes in the secondary structure of dental biofilm proteins in people with cariesogenic and cariesoprotective status using synchrotron IR microspectroscopy.

Materials And Methods: Dental biofilm samples from 50 patients without caries (group 1) and with carious lesions of tooth enamel (group 2) were studied. The molecular composition of biofilms was studied using IR microspectroscopy.

A Study of the Association between Primary Oral Pathologies (Dental Caries and Periodontal Diseases) Using Synchrotron Molecular FTIR Spectroscopy in View of the Patient's Personalized Clinical Picture (Demographics and Anamnesis).

In this exploratory study, we searched for associations between the two most common diseases of the oral cavity-dental caries and periodontal diseases-taking into account additional factors, such as personalized clinical pictures (the individual risk factors of the patient), based on the method of a multivariate data analysis of the molecular changes in the composition of human gingival crevicular fluid (GCF). For this purpose, a set of synchrotron Fourier-transform infrared spectroscopy (FTIR) spectra of gingival crevicular fluid samples from patients with different demographics, levels of dental caries development and periodontal diseases, and the presence/absence of concomitant chronic diseases were obtained and analyzed. Using a set of techniques (v-, F-, Chi-square tests; a principal component analysis (PCA); and the hierarchical clustering of principal components (HCPCs)) implemented in the R package FactoMineR allowed us to assess the relationship between the principal components (PCs) and characteristics of the respondents.

Rapid Deposition of the Biomimetic Hydroxyapatite-Polydopamine-Amino Acid Composite Layers onto the Natural Enamel.

In this work, we developed a technology that enables rapid deposition of biomimetic composite films onto natural enamel slices (known as biotemplates). These films are composed of polydopamine (PDA) and nanocrystalline carbonate-substituted hydroxyapatite (nano-cHAp) that have been functionalized with amino acid l-Arginine. We utilized atomic force microscopy (AFM) and scattering scanning near-field optical microscopy (s-SNOM) combined with infrared (IR) synchrotron to achieve nanoscale spatial resolution for both IR absorption and topography analyses.

[Organization of maxillofacial and dental care for the wounded of the Voronezh front (July 1942 - July 1943)].

The article is devoted to the history of the formation of the system of assistance to maxillofacial wounded soldiers of the Voronezh Front during the battles for Voronezh in 1942-1943. The difficulties and achievements in the implementation of phased assistance to the wounded in the face, as well as the improvement of the organizational structure of surgical and dental care for soldiers and officers of the front are reflected.

Changes in Dental Biofilm Proteins' Secondary Structure in Groups of People with Different Cariogenic Situations in the Oral Cavity and Using Medications by Means of Synchrotron FTIR-Microspectroscopy.

This work unveils the idea that the cariogenic status of the oral cavity (the presence of active caries lesions) can be predicted via a lineshape analysis of the infrared spectral signatures of the secondary structure of proteins in dental biofilms. These spectral signatures that work as natural markers also show strong sensitivity to the application in patients of a so-called modulator-a medicinal agent (a pelleted mineral complex with calcium glycerophosphate). For the first time, according to our knowledge, in terms of deconvolution of the complete spectral profile of the amide I and amide II bands, significant intra- and intergroup differences were determined in the secondary structure of proteins in the dental biofilm of patients with a healthy oral cavity and with a carious pathology.

A Study of the Peculiarities of the Formation of a Hybrid Interface Based on Polydopamine between Dental Tissues and Dental Composites, Using IR and Raman Microspectroscopy, at the Submicron Level.

The creation of buffer (hybrid) layers that provide improved adhesion to two heterogeneous materials is a promising and high-priority research area in the field of dental materials science. In our work, using FTIR and Raman microspectroscopy at the submicron level in a system of dental composites/intact dental enamel, we assessed the molecular features of formation and chemically visualized the hybrid interface formed on the basis of a nature-like adhesive, polydopamine (PDA). It is shown that a homogeneous bioinspired PDA-hybrid interface with an increased content of O-Ca-O bonds can be created using traditional methods of dental tissue pretreatment (diamond micro drilling, acid etching), as well as the subsequent alkalinization procedure and the developed synthesis technology.

[Distinctions in molecular composition of the dental biofilm depending on the method of exo-/endogeneous caries prevention and cariogenic condition of a patient].

Purpose Of The Study: For the first time distinctions of molecular composition of the dental biofilm at the stages of exo- and endogeneous caries prevention were studied for persons with different cariogenic conditions involving synchrotron molecular spectroscopy techniques.

Material And Methods: The samples of the dental biofilm collected from participants of the research were studied at the different stages of experiment. The studies of molecular composition of the biofilms were employed involving the equipment set in the Infrared Microspectroscopy (IRM) laboratory of Australian synchrotron.

Development of a Hybrid Biomimetic Enamel-Biocomposite Interface and a Study of Its Molecular Features Using Synchrotron Submicron ATR-FTIR Microspectroscopy and Multivariate Analysis Techniques.

Using a biomimetic strategy and bioinspired materials, our work proposed a new technological approach to create a hybrid transitional layer between enamel and dental biocomposite. For this purpose, an amino acid booster conditioner based on a set of polar amino acids (lysine, arginine, hyaluronic acid), calcium alkali, and a modified adhesive based on BisGMA and nanocrystalline carbonate-substituted hydroxyapatite are used during dental enamel restoration. The molecular properties of the hybrid interface formed using the proposed strategy were understood using methods of multivariate statistical analysis of spectral information collected using the technique of synchrotron infrared microspectroscopy.

Biomimetic Mineralization of Tooth Enamel Using Nanocrystalline Hydroxyapatite under Various Dental Surface Pretreatment Conditions.

In this report, we demonstrated the formation of a biomimetic mineralizing layer obtained on the surface of dental enamel (biotemplate) using bioinspired nanocrystalline carbonate-substituted calcium hydroxyapatite (ncHAp), whose physical and chemical properties are closest to the natural apatite dental matrix, together with a complex of polyfunctional organic and polar amino acids. Using a set of structural, spectroscopy, and advanced microscopy techniques, we confirmed the formation of a nanosized ncHAp-based mineralized layer, as well as studying its chemical, substructural, and morphological features by means of various methods for the pretreatment of dental enamel. The pretreatment of a biotemplate in an alkaline solution of Ca(OH) and an amino acid booster, together with the executed subsequent mineralization with ncHAp, led to the formation of a mineralized layer with homogeneous micromorphology and the preferential orientation of the ncHAp nanocrystals.

Effect of Exo/Endogenous Prophylaxis Dentifrice/Drug and Cariogenic Conditions of Patient on Molecular Property of Dental Biofilm: Synchrotron FTIR Spectroscopic Study.

(1) Objectives: This study is the first one to investigate the molecular composition of the dental biofilm during the exogenous and endogenous prophylaxis stages (use of dentifrice/drug) of individuals with different cariogenic conditions using molecular spectroscopy methods. (2) Materials and Methods: The study involved 100 participants (50 males and 50 females), aged 18-25 years with different caries conditions. Biofilm samples were collected from the teeth surface of all participants.

Development of a Visualisation Approach for Analysing Incipient and Clinically Unrecorded Enamel Fissure Caries Using Laser-Induced Contrast Imaging, MicroRaman Spectroscopy and Biomimetic Composites: A Pilot Study.

This pilot study presents a practical approach to detecting and visualising the initial forms of caries that are not clinically registered. The use of a laser-induced contrast visualisation (LICV) technique was shown to provide detection of the originating caries based on the separation of emissions from sound tissue, areas with destroyed tissue and regions of bacterial invasion. Adding microRaman spectroscopy to the measuring system enables reliable detection of the transformation of the organic-mineral component in the dental tissue and the spread of bacterial microflora in the affected region.

The Molecular and Mechanical Characteristics of Biomimetic Composite Dental Materials Composed of Nanocrystalline Hydroxyapatite and Light-Cured Adhesive.

The application of biomimetic strategies and nanotechnologies (nanodentology) has led to numerous innovations and provided a considerable impetus by creating a new class of modern adhesion restoration materials, including different nanofillers. An analysis of the molecular properties of biomimetic adhesives was performed in this work to find the optimal composition that provides high polymerisation and mechanical hardness. Nanocrystalline carbonate-substituted calcium hydroxyapatite (nano-cHAp) was used as the filler of the light-cured adhesive Bis-GMA (bisphenol A-glycidyl methacrylate).

[Biomimetic strategy of a hybrid biointerface creation between native human dental tissue and dental composite].

Objective: The aim of the studyis the peculiarities of the molecular composition of the biointerface between the native human dental tissue and the dental composite, created in an alkaline environment, based on synchrotron chemical infrared micro-mapping.

Material And Methods: When creating a biomimetic interface under alkaline conditions, we used an original bioprimer, dentin conditioner, nanofilled universal adhesive, and a light-curing compomer based on BIS-GMA.

Results: Biointerface analysis was carried out on the basis of chemical infrared micro-mapping, implemented using the equipment of the Australian Synchrotron, and subsequent multivariate cluster analysis of the collected spectral data array.

Raman and XANES Spectroscopic Study of the Influence of Coordination Atomic and Molecular Environments in Biomimetic Composite Materials Integrated with Dental Tissue.

In this work, for the first time, the influence of the coordination environment as well as Ca and P atomic states on biomimetic composites integrated with dental tissue was investigated. Bioinspired dental composites were synthesised based on nanocrystalline calcium carbonate-substituted hydroxyapatite Ca4ICa6IIPO46-xCO3x+yOH2-y (nano-cHAp) obtained from a biogenic source and a set of polar amino acids that modelled the organic matrix. Biomimetic composites, as well as natural dental tissue samples, were investigated using Raman spectromicroscopy and synchrotron X-ray absorption near edge structure (XANES) spectroscopy.

To the Question on the Use of Multivariate Analysis and 2D Visualisation of Synchrotron ATR-FTIR Chemical Imaging Spectral Data in the Diagnostics of Biomimetic Sound Dentin/Dental Composite Interface.

In this short communication, we provide information on the use of the hierarchical cluster analysis of synchrotron ATR-FTIR 2D chemical imaging spectral data as a useful and powerful approach to the microspectroscopic diagnostics of molecular composition in the hybrid sound dentin/dental composite interfaces and materials, including ones developed with the use of biomimetic strategies. The described diagnostic approach can be successfully transferred to the analysis and visualisation of 2D spectral data, collected using laboratory Raman and FTIR microspectroscopy techniques.

Engineering of a Biomimetic Interface between a Native Dental Tissue and Restorative Composite and Its Study Using Synchrotron FTIR Microscopic Mapping.

The aim of this work is to develop a biomimetic interface between the natural tooth tissue and the restorative composite and to study it on the basis of synchrotron micro-FTIR mapping and multidimensional processing of the spectral data array. Using hierarchical cluster analysis of 3D FTIR data revealed marked improvements in the formation of the dentine/adhesive/dental hybrid interface using a biomimetic approach. The use of a biomimetic strategy (application of an amino acid-modified primer, alkaline calcium and a nano-c-HAp-modified adhesive) allowed the formation of a matrix that can be structurally integrated with natural dentine and dental composite.

Development of a new approach to diagnosis of the early fluorosis forms by means of FTIR and Raman microspectroscopy.

This study is aimed at investigating the features of mineralization of the enamel apatite at initial stages of fluorosis development. Samples of teeth with intact and fluorotic enamel in an early stage of the disease development (Thylstrup-Fejerskov Index = 1-3) were studied by Raman scattering and FTIR using Infrared Microspectroscopy beamline at Australian Synchrotron equipment. Based on the data obtained by optical microspectroscopy and calculation of the coefficient R [A-type/B-type], which represents the ratio of carbonation fraction of CO, replacing phosphate or hydroxyl radicals in the enamel apatite lattice, the features of mineralization of enamel apatite in the initial stages of development of the pathology caused by an increased content of fluorine in the oral cavity were established.

[Spectroscopic studies of the molecular composition of the dentinal and gingival fluids and their diagnostic potential for preventive screening of dentin caries].

Purpose Of The Study: The aim of the work is a spectroscopic investigation and comparison of the molecular composition of dentine and gingival fluids as well as of their diagnostic potential for the preventive screening of dentin caries.

Material And Methods: Applying infrared spectroscopy including synchrotron radiation the samples of biological fluids taken from the oral cavity (dentine fluid, fluid from the gingival sulcus and blood) were studied for the patients with the progressive dentine caries. The part of this research was undertaken with the infrared microspectroscopy beamline at the Australian Synchrotron.

Organic-Mineral Interaction between Biomimetic Materials and Hard Dental Tissues.

Unlabelled: was to study the integration between native human dental tissue and new-generation biomimetic materials replicating the mineral-organic complex of dentin and enamel using IR microspectroscopy for multidimensional visualization and analysis.

Materials And Methods: The conditions for stable integration at the interface between biomimetic material and natural hard tissue were identified using a biocomposite buffer system of nanocrystalline carbonate-substituted calcium hydroxyapatite corresponding in its total characteristics to human dentin-enamel apatite and a number of amino acids present in the organic matrix of dentin and enamel: L-histidine, L-lysine hydrochloride, L-arginine hydrochloride, and hyaluronic acid. The finished samples were studied using IR microspectroscopy on IRM channel equipment (The Australian Synchrotron, Melbourne, Australia).

[Spectroscopic studies of the changes occurring in the molecular composition of oral fluid in people with multiple caries].

The changes in molecular composition of mixed saliva (oral fluid) were analyzed with the use of spectroscopic techniques for the patients with multiple caries. Using Fourier-transform infrared spectroscopy studies including synchrotron radiation for the excitation certain features were detected in the IR-spectra of the oral fluid. Mineral-organic, carbon-phosphate, AmidII/AmidI and protein/thiocyanate ratios were calculated for the groups of patients with multiple caries and patients of the control group.

Spectroscopic signature of the pathological processes of carious dentine based on FTIR investigations of the oral biological fluids.

The aim of our work is to find a spectroscopic signature of the pathological processes of carious dentine based on the investigations of the molecular composition of the oral biological fluids with the use of FTIR synchrotron techniques. This complex analysis of the obtained data shows that a number of signatures are present only in the spectra of dentine and gingival fluids from the patients developing caries of the deep dentine tissues. The detected features and complex analysis of the quantitative and qualitative data representing signatures of the development of oral cavity pathologies can enhance the quality of dental screening.

Pathology-specific molecular profiles of saliva in patients with multiple dental caries-potential application for predictive, preventive and personalised medical services.

Background: Improving the quality of life is part of the global agenda. The focus is predominantly on prevention of socially significant diseases. Combating dental caries-related diseases is a top priority as it has a huge impact on people's social lives.

[Increased oral fluid remineraling function by endogenous and exogenous saturation methods of its mineral complexes].

Relevance: One of the most common problems in modern caries prevention is the development of techniques to increase tooth resistance to the effects of cariogenic factors. Therefore, research is needed to study the chemical composition of tissues like teeth and body fluids surrounding the tooth to prevent demineralization process. The purpose of determining the effectiveness of work- and correlation relationship between endo saturation exogenous methods oral liquid mineral complexes.

[MICROFLORA AND ORAL DISEASE].

Acid-producing microorganisms are base etiological agents of lesions of tooth enamel and destruction of dentin. The process start by specific microflora of tooth deposit--Streptococcus mutans, Lactobacteria and Actinomycetis viscosus which ferment food carbogydrate to form acids. High titre of them in oral cavity may be considered like a marker of carbohydrate food.