Zh Mikrobiol Epidemiol Immunobiol
April 1992
The first Soviet kits for the serological identification of streptococci, groups A, B, and C, on the basis of the coagglutination test were developed. Each kit was intended for 35-40 determinations. The optimum concentration of streptococci during their identification by means of the reagents making up the kit was about 1.
View Article and Find Full Text PDFZh Mikrobiol Epidemiol Immunobiol
February 1993
The present work deals with trials of the method of rapid diagnosis of streptococcal infection, carried out in children's infectious hospital, with the use of a new diagnostic kit. The proposed diagnosticum has proved to be highly sensitive and specific in scarlet fever and tonsillitis. The sensitivity and specificity of the diagnosticum depend on the duration of the disease, prehospital treatment and the quality of the bacteriological analysis.
View Article and Find Full Text PDFZh Mikrobiol Epidemiol Immunobiol
June 1993
The study revealed that the isolation rate of group A streptococci in scarlet fever patients at the time of hospitalization did not exceed 68%. The isolation rate of these streptococci was greatly influenced by antibacterial therapy carried out before hospitalization. Under clinical conditions with intensive penicillin therapy group A streptococci were eliminated from the larynx on days 3-4.
View Article and Find Full Text PDFBacteriological confirmation of the presence of group A Streptococcus (GAS) in patients with acute rheumatic fever (ARF) by means of the express method was achieved only in 15.1% of the cases and the positive growth of the culture was observed only in 12.1%.
View Article and Find Full Text PDF44 autopsy cases (from 1985 to 1990) are studied out of which in 38 cases the diseases were produced by streptococcus of group A. In 18 cases there was a pharyngeal or extra-pharyngeal generalized streptococcus infection (scarlet fever). Moderately pronounced local damage, mainly pneumonia, were found in 20 cases.
View Article and Find Full Text PDFZh Mikrobiol Epidemiol Immunobiol
December 1990
The work deals with the development of the rapid method of the identification of acute streptococcal infection on the basis of the coagglutination test. The rapid method of the extraction of group-specific polysaccharide antigen from the cell walls of group A streptococci is proposed. The data on the use of native sera and their fractions in the development of coagglutination diagnostica have been described and analyzed.
View Article and Find Full Text PDFZh Mikrobiol Epidemiol Immunobiol
July 1990
The influence of the OF activity of group A streptococci on their specific pathogenic properties has been shown, which is manifested by increased virulence of these streptococci for children of younger age groups and by a two times higher isolation rate of OF+ strains in tonsillitis than in scarlet fever. The possibility of the indirect evaluation of the content of anti-M-antibodies by the results of the anti-OF test has been revealed, which permits using this test instead of the bactericidal test, more complicated, in the study of immunity to infection induced by group A OF+ streptococci. Among the main methods of laboratory support of epidemiological surveillance on streptococcal infection, the introduction of the highly discriminating OF typing and the anti-OF test into practical use is recommended.
View Article and Find Full Text PDFThe dynamics of scarlet fever morbidity in Leningrad for many years was studied and the absence of any effect produced by the existing system of preventive measures against this disease for the last 30 years was shown. On the basis of epidemiological and bacteriological data the authors came to the conclusion on the unity of the epidemic process of scarlet fever in the whole city and the relatively autonomous character of the process in individual districts. The necessity of the realization of epidemiological surveillance on streptococcal infection, with special emphasis on the multilevel social structure of the city and the wide use of the bacteriological diagnosis of streptococcal diseases, is substantiated.
View Article and Find Full Text PDFZh Mikrobiol Epidemiol Immunobiol
September 1986
In the serum samples obtained from residents of the Todzhinsky district in the Tuva Autonomous Soviet Socialist Republic antibodies to 12 out of 15 studied types of group A streptococcal lipoproteinases (OF-factor) were detected, with the prevalence of types 2, 4, 22, 25, 48, and 60, their incidence in the population constituting 34%. Antibodies to OF-antigens 58, 62, and 63 were not detected. The distribution of OF-antibodies was found to vary with sex, occurring in females 1.
View Article and Find Full Text PDFMol Gen Mikrobiol Virusol
March 1985
A system for insertion of genes into the chromosome of group H streptococci has been elaborated. It consists of a recipient strain (Gallis GS10/1), having the fragments of lambda L-47-1 bacteriophage DNA inserted into the chromosome, and lambda 202 vector. The constructed system suggests the preliminary cloning of genes in E.
View Article and Find Full Text PDFBy using electron microscopy of self-annealed DNA and restriction enzyme analysis, we have compared the physical maps of two group A streptococcal plasmids associated with conventional MLS resistance (pEL1; 20 Md) and zonal lincomycin resistance (pSM10419; 15 Md). Of their monomeric molecules, about 40% and 60%, respectively, are occupied by identical non-tandem inverted repeats containing sequences specifying putative replication functions. Sequence homology also exists between their resistance determinants which are located in unique DNA.
View Article and Find Full Text PDFDNA of the streptococcal plasmid ERLI, determining resistance to erythromycin and lincomycin has been studied. The presence of satellite DNA component in streptococcal DNA has been demonstrated by dye-CsCl and CsCl density centrifugation, by chromatography of denatured-renatured DNA on the nitrocellulose and by electron microscopy. By all these methods the presence of covalently closed circular DNA molecules has been shown.
View Article and Find Full Text PDFZentralbl Bakteriol Orig A
December 1975
The possibility of revealing lipoproteinase (serum opacity factor--OF) in hydrochloric and alkaline extracts from streptococcal cultures and the possibility of concentration and purification of this enzyme by means of gel-filtration and ion-exchange chromatography have been confirmed. When studying antibodies to OF in the blood of healthy and sick people, we were able both to reveal and to determine the titre of antibodies to OF of different serological M types (2, 4, 22/12, 22 and 49) in the sera under study (170 sera from the same number of persons). In groups of healthy people, anti-OF antibodies were shown to be present with a high frequency as well as in patients.
View Article and Find Full Text PDFZh Mikrobiol Epidemiol Immunobiol
August 1975
In examining 170 samples of blood sera of healthy and sick individuals it was shown that with the aid of a simple and express antilipoproteinase test it was possible not only to reveal, but also to determine the titre of specific antibodies to the lipoproteinases of various serological M-types (2, 4, 22/12, 22 and 49) of a hemolytic streptoccus, group A. The antibodies to the enzymes were frequently revealed in the sera of adults and older children. The results obtained were still inadequate for the epidemiological or clinico-immunological analysis, but pointed to the principal possibility of using the antilipoproteinase test for the mentioned purpose.
View Article and Find Full Text PDFZh Mikrobiol Epidemiol Immunobiol
July 1974
Zh Mikrobiol Epidemiol Immunobiol
March 1972