The high-intensity reflectometer of the Jülich Centre for Neutron Science: MARIA.

MARIA (magnetism reflectometer with high incident angle) is a world class vertical sample reflectometer dedicated to the investigation of thin films in the fields of magnetism, soft matter and biology. The elliptical vertically focusing guide allows one to measure small samples with a typical size of 1 × 1 cm very efficiently. The double-bounce polarizer and the pumped He SEOP (spin-exchange optical pumping) neutron spin filter cell for analysing the polarization of the reflected neutron beam can be moved into the beam in seconds.

Studies on the adsorption and desorption of mitoxantrone to lauric acid/albumin coated iron oxide nanoparticles.

A rational use of superparamagnetic iron oxide nanoparticles (SPIONs) in drug delivery, diagnostics, and other biomedical applications requires deep understanding of the molecular drug adsorption/desorption mechanisms for proper design of new pharmaceutical formulations. The adsorption and desorption of the cytostatic Mitoxantrone (MTO) to lauric acid-albumin hybrid coated particles SPIONs (SEON) was studied by Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), surface titration, release experiments and small-angle neutron and X-ray scattering. Such MTO-loaded nanoparticles have shown very promising results in in vivo animal models before, while the exact binding mechanism of the drug was unknown.

[Bactericidal effect of lysozyme].

Isolation of lysozyme from hemolymph of Alveonasus lahorensis (Acari: Parasitiformes, Argasidae) and Hyalomma marginatum (Acari: Parasitiformes, Ixodidae) with using ultrasound is described. It was shown that the bactericidal effect of the ultrasound-extracted lysozyme against Staphylococcus aureus and Micrococcus luteus significantly exceeded that of the chicken egg lysozyme and lysozyme from ticks without ultrasound exposure. Disintegration of the hemolymph cells increased lysozyme production.

[Laparoscopic intraabdominal hernioplasty as the operation of choice in the inguinal hernia treatment].

Comparative analysis of a pain syndrome severity was done, using a visual analogous scale (VAS), basing on the analysis of performance of various kinds of laparoscopic prosthesis inserting hernioplasty for inguinal hernia, using intraabdominal (intraperitoneal onlay mesh - IPOM) and preperitoneal (transabdominal preperitoneal - TAPP) with establishment of light nets. A pain severity is trustworthy lesser in the IPOM method application--5 and 17.5 points, accordingly.

The low resolution structure of ApoA1 in spherical high density lipoprotein revealed by small angle neutron scattering.

Spherical high density lipoprotein (sHDL), a key player in reverse cholesterol transport and the most abundant form of HDL, is associated with cardiovascular diseases. Small angle neutron scattering with contrast variation was used to determine the solution structure of protein and lipid components of reconstituted sHDL. Apolipoprotein A1, the major protein of sHDL, forms a hollow structure that cradles a central compact lipid core.

Congruency between biophysical data from multiple platforms and molecular dynamics simulation of the double-super helix model of nascent high-density lipoprotein.

The predicted structure and molecular trajectories from >80 ns molecular dynamics simulation of the solvated Double-Super Helix (DSH) model of nascent high-density lipoprotein (HDL) were determined and compared with experimental data on reconstituted nascent HDL obtained from multiple biophysical platforms, including small angle neutron scattering (SANS) with contrast variation, hydrogen-deuterium exchange tandem mass spectrometry (H/D-MS/MS), nuclear magnetic resonance spectroscopy (NMR), cross-linking tandem mass spectrometry (MS/MS), fluorescence resonance energy transfer (FRET), electron spin resonance spectroscopy (ESR), and electron microscopy. In general, biophysical constraints experimentally derived from the multiple platforms agree with the same quantities evaluated using the simulation trajectory. Notably, key structural features postulated for the recent DSH model of nascent HDL are retained during the simulation, including (1) the superhelical conformation of the antiparallel apolipoprotein A1 (apoA1) chains, (2) the lipid micellar-pseudolamellar organization, and (3) the solvent-exposed Solar Flare loops, proposed sites of interaction with LCAT (lecithin cholesteryl acyltransferase).

[Quality of life in patients after restorative operations].

The analysis of the results of the surgical treatment of colon cancer, ulcerative colitis, Crohn's disease, polyposis of colon, diverticular disease of colon on the bases of quality of life scale indexes, questionnaire SF-36 was carried out. The authors have compared the quality of life of patients who underwent surgical treatment using restorative operations after removing functional parts of the colon. The quality of life indexes in 11-12 days after the operation were higher in the first group compared with the second one.

[Methods of Barrett disease diagnostics].

Chromoesophagoscopy and photodynamic diagnosis are objective methods for diagnostic Barrett disease. Those methods can give us more detailed information about morphological changes in mucosa of esophagus.

[Surgical tactics in the ulcer pyloroduodenal stenosis in decompensation stage].

In the ulcer pyloroduodenal stenosis the stages of complete and noncomplete decompensation are suggested. In 73% of observations, while performing an adequate preoperative preparation it was possible to convert pyloroduodenal stenosis from the stage of complete decompensation into the stage of noncomplete decompensation, in which the performance of organpreserving operation is possible. In the patients, suffering pyloroduodenal stenosis in the stage of noncomlete decompensation, resection of 2/3 of stomach is performed.

[The prognostic factors of the colonic cancer occurrence in nonspecific ulcerative colitis].

The prognostic factors of the colonic cancer occurrence in nonspecific ulcerative colitis were determined. Photodynamical diagnosis of the colonic mucosa affection, immunohistochemical determination of proliferation and apoptosis markers were applied in the investigation. The statistic-probability pattern of the malignization processes dynamics was elaborated.

[The choice of tactics in the treatment of nonspecific ulcerative colitis].

Taking into account high probability of occurrence of the colonic mucosa morphological atypia in nonspecific ulcerative colitis, the methods of trustworthy diagnosis of dysplasia determine the choice of the treatment tactics as well as the operative intervention volume. The methods of chromocolonoscopy and photodynamical diagnosis of nonspecific ulcerative colitis are presented, their role in the treatment of patients tactic and considering the operative intervention volume was determined.

[Comparative immunohistochemical features of Crohn disease and ulcerative colitis].

The determination of markers of proliferation and apoptosis in cases of Crohn disease and ulcerative colitis enables to foresee the development of morphological pathological changes occurred in a colon mucosa.

[Primary chemoprevention of colorectal cancer in patients with inflammatory bowel diseases].

The article discusses current situation with colorectal cancer and inflammatory bowel diseases--ulcerative colitis and Crohn's desease. It has presented modern strategies of prevention colorectal cancer in this pathology and own data about the efficacy chemoprevention of cancer with 5-ASA.

[Selection of methods of reconstructive operations in surgical treatment of unspecific ulcerative colitis].

Chromocolonoscopic diagnostics in recognizing of displastics lisions of mucosa is described in the article. The indications for different methods of reconstructive operations are presented.

[Gastroesophageal reflux disease as a surgical problem].

Photodynamical and chromoesophagoscopic diagnostics in recognizing of Barrett disease are described in the article. The indications for surgical treatment of esophagogastric reflux disease are presented.

[Efficacy of Norgalax and Normacol in preparation for examination of the large bowel].

The article contains the results of the prospective non-controlled study of norgalax and normacol enemas in preparation to endoscopic and radiological examinations of the large bowel. The results of investigation showed high effectiveness, simple use of enemas which can be recommended for preparation to examination of and surgery on the large bowel.

Ioffe et Al. Reply:

A Reply to the Comment by C. A. Chatzidimitriou-Dreismann et al.