Publications by authors named "Ioannis Manousakas"

Article Synopsis
  • - Since the COVID-19 pandemic, mental health issues have surged, prompting the need for effective monitoring using wearable devices and mobile health apps that track heart rate variability (HRV) through electrocardiogram (ECG) measurements.
  • - This study introduces a new single-arm ECG measurement technique that simplifies the process by only needing one electrode instead of the usual two, making it easier to gather vital mental health data.
  • - Experiments showed that the new single-arm ECG method closely correlates with standard lead I ECG readings, particularly in heart rate interval measurements, and could provide reliable insights into mental health conditions with better practical application.
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Purpose: This study aimed to develop a quantitative dry cupping system that can monitor negative pressure attenuation and soft tissue pull-up during cupping to quantify soft tissue compliance.

Methods: Baseball players with myofascial pain syndrome were recruited to validate the benefits of cupping therapy. Nine of 40 baseball players on the same team were diagnosed with trapezius myofascial pain syndrome; another nine players from the same team were recruited as controls.

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Astaxanthin (Asta) has been demonstrated to possess anti-inflammatory, antitumor, and free radical-clearing activities. However, the poor stability and low water solubility of Asta hamper its bioavailability. The objectives of this study were to fabricate Asta-loaded liposomes (Asta-lipo) and investigate the therapeutic effects of Asta-lipo on alcoholic liver fibrosis in mice.

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Volvox sphere is a bio-mimicking concept of a biomaterial structure design able to encapsulate chemicals, drugs and/or cells. The aim of this study was to prepare Volvox spheres encapsulating AML12 liver cells and mesenchymal stem cells (MSCs) via a high voltage electrostatic field system. The results demonstrated that AML12 liver cells and MSCs could be successfully encapsulated into the inner spheres and the outer sphere of the Volvox spheres.

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Article Synopsis
  • - The study investigates a specialized 3D culture system called Volvox spheres, designed to effectively co-culture mesenchymal stem cells (MSCs) and AML12 liver cells.
  • - Findings indicate that MSCs can transform into liver-like cells when cultured in Volvox spheres, exhibiting key liver cell markers and proteins relevant to liver function.
  • - The type of growth media used (DMEM vs. DMEM/F12) significantly influences MSC behavior, with different peaks in marker expression observed at various points during the 14-day culture period.
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Background: Low-intensity pulsed ultrasound (LIPUS) regiment has been used to treat fractures with non-union and to promote bone union in general. The effect of LIPUS on articular cartilage metabolism has been characterized. Yet, the effect of LIPUS to repair articular cartilage injury remains unclear in vivo.

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Volvox sphere is a bio-mimicking concept of an innovative biomaterial structure of a sphere that contains smaller microspheres which then encapsulate chemicals, drugs and/or cells. The volvox spheres were produced via a high-voltage electrostatic field system, using alginate as the primary material. Encapsulated materials tested in this study include staining dyes, nuclear fast red and trypan blue, and model drugs, bovine serum albumin (BSA) and cytochrome c (CytC).

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Extracorporeal Shock Wave Lithotriptors are very popular for the treatment of urinary stones all over the world. They depend basically upon either X-ray fluoroscopy or ultrasound scans to detect the stones before therapy begins. To increase the effectiveness of treatment this study took advantage of both X-ray and ultrasound to develop a dual stone locating system with image processing modules.

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Purpose: Extracorporeal shock wave lithotripsy has been popular since the 1980s. Only 30% to 50% of the shock waves of all conventional lithotripters are focused on stones. We developed an ultrasound based, real-time stone tracking system (version 1) to improve accuracy and treatment efficiency.

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Collagen II and hyaluronan are the two major components of the native extracellular matrix (ECM). Both biopolymers are responsible for providing the associated tissues with tensile strength, and also serve as a structural scaffold for cell adhesion and growth. Over the years, many researchers have focused on the preparation and evaluation of man-made ECM comprising the two polymers in the form of a membrane for chondrocyte culture applications.

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Although extracorporeal shock wave lithotripsy (ESWL) is the standard procedure for treating renal stones, little work has been done on ways to minimize the side effects and on treatment optimization. A system that uses ultrasound imaging for real time renal stone monitoring is presented here and some aspects of the image analysis module are described. The improved system has proved to be more robust than the unmodified system.

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