Publications by authors named "Ioannis Liaskas"
Article Synopsis
- Modern live imaging techniques, such as light-sheet fluorescence microscopy (LSFM), allow for detailed lineage tracing of cells, revealing their development and behavior in real time.
- LSFM enables imaging of whole organisms over extended periods while minimizing damage to the specimens, significantly enhancing our understanding of cellular dynamics.
- The chapter outlines how to prepare LSFM datasets and introduces three software platforms (MaMuT, Mastodon, and TrackMate) for tracking cell lineages in a crustacean model, Parhyale hawaiensis, through various tracking methods.
We present the application of a low-cost frequency domain photoacoustic (FDPA) microscope for the label-free imaging of live developing embryos of the crustacean model organism Parhyale hawaiensis. By modulating the intensity of a continuous wave laser source at 9.5 MHz, we achieve the excitation of monochromatic PA waves, which are detected to provide amplitude and phase recordings.
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