A retrospective study of the aetiology of increased mean platelet volume in dogs presented to a small animal teaching hospital in Greece.

Background: The aetiology of increased mean platelet volume in dogs is currently uncertain. Our aim was to investigate the aetiology of increased mean platelet volume in dogs with and without thrombocytopenia.

Methods: The database of a teaching hospital was retrospectively searched for dogs with increased mean platelet volume (>14.

Glycated haemoglobin and serum fructosamine concentrations in sick, non-diabetic dogs receiving oral prednisolone.

Background: Glucocorticoids have been associated with an increased risk of developing diabetes mellitus (DM) in dogs. Glycated haemoglobin (HbA1c) and serum fructosamine have been scarcely studied in dogs receiving glucocorticoid therapy. The aim of this study was to evaluate HbA1c and fructosamine in non-diabetic dogs receiving oral prednisolone.

A comparison study between the Siemens ADVIA 120 and manual method for the differential white blood cell count in goats.

Background: Although widely used, the ADVIA 120 hematology analyzer has not been previously validated for determining the differential leukocyte count in goats.

Objectives: The aim of this study was to compare the differential leukocyte counts provided by the ADVIA 120 (A-diff) and the manual method (M-Diff) in goats.

Methods: EDTA blood samples that were analyzed within 4 h of collection were used in the study.

Association of platelet indices with glycemic status in diabetic dogs.

We investigated the platelet count (PLT), mean platelet volume (MPV), and plateletcrit (PCT) in dogs with type 1 diabetes mellitus (DM) compared to healthy controls, and their association with the major fraction of glycated hemoglobin (HbA1c). Blood samples from 33 clinically healthy dogs and 14 newly diagnosed diabetic dogs were included. CBCs were performed with the Advia 120; HbA1c was determined using a validated assay (Capillarys 2 flex-piercing; Sebia).

The effect of age and sex on glycated hemoglobin in dogs.

We investigated the effect of age and sex on canine glycated hemoglobin (HbA1c) using a validated capillary electrophoresis assay. Aliquots of EDTA blood samples collected for routine health checks were used. HbA1c was measured using the Capillarys 2 flex-piercing system (Sebia).

Diagnostic and prognostic value of peripheral blood leucocyte ratios in sick cats.

The objective of this study was to assess the diagnostic and prognostic utility of feline neutrophil-to-lymphocyte (NLR) and lymphocyte-to-monocyte ratios (LMR) in a variety of underlying diseases. Five-year medical records from cats presenting to the internal medicine unit of a veterinary teaching hospital were retrospectively reviewed. Cats were considered for inclusion based on complete medical records.

Effect of anaemia and erythrocyte indices on canine glycated haemoglobin.

Background: The major fraction of glycated haemoglobin (HbA1c) depends on blood glucose concentration and erythrocyte lifespan, and consequently erythrocyte indices may affect HbA1c; our objective was to study this effect in dogs.

Methods: Blood samples from two (healthy and anaemic) age- and sex-matched, normoglycaemic populations were prospectively included. Advia 120 and Capillarys 2 flex-piercing were used for the haematological and HbA1c analyses, respectively.

Investigation of hypomagnesaemia prevalence and underlying aetiology in a hospitalised cohort of dogs with ionised hypocalcaemia.

Objectives: Calcium is the most abundant mineral in the body and plays a critical role in a wide range of physiological processes. Low concentrations of ionised calcium, the most metabolically available form of calcium, have been linked to an increased risk of adverse clinical outcomes in dogs. Magnesium plays an important role in parathyroid hormone function.

A comparison study between the results of the Siemens ADVIA 120 analyzer and the manual method for differential leukocyte counts in sheep.

Background: The ADVIA 120 is a widely used hematology analyzer, which has not been previously validated for determining differential leukocyte counts in sheep.

Objectives: We aimed to compare differential leukocyte counts on the ADVIA 120 (A-Diff) with counts obtained using the manual method (M-Diff) in sheep.

Methods: Ethylenediaminetetraacetic acid-anticoagulated blood samples analyzed within 4 hours of collection were used.

Effects of surgery on free and total 25 hydroxyvitamin D concentrations in dogs.

Background: It is unclear whether a low total 25(OH)D concentration is a cause or consequence of illnesses. To address this knowledge gap, studies measuring free and total 25(OH)D during the evolution and resolution of an inflammatory process are required.

Objectives: Serum total and free 25(OH)D concentrations would transiently decline after cruciate surgery in dogs.

Capillary hemoglobin electrophoresis of healthy and anemic dogs: Quantification, validation, and reference intervals of hemoglobin fractions.

Despite the advances in canine medicine and the rapid gaining of attention of canine models in biomedical field and particularly in hemoglobin genes research, the studies on canine hemoglobin composition are sparse with ambiguous findings. Our aim was: i) to investigate the electrophoretic pattern of canine hemoglobin and the possible effects of age, sex, and anemia using a capillary electrophoresis assay, and ii) to validate this assay and calculate reference intervals (RIs) for canine hemoglobin fractions. Blood samples were collected from 53 healthy and 42 dogs with regenerative and non-regenerative anemias.

Circulating and bone marrow myeloid cells containing amastigotes in a case of advanced canine leishmaniosis.

A 5-y-old male Poodle mix was presented with intermittent vomiting, anorexia, and weight loss. Physical examination revealed emaciation, lethargy, dehydration, hypothermia, respiratory distress, and splenomegaly. Based on clinicopathologic, serologic, and parasitologic findings, diagnoses of severe leishmaniosis and dirofilariasis were made.

Hereditary methemoglobinemia in a cyanotic cat presented for ovariohysterectomy.

A 1-year-old, female, domestic shorthair cat with a history of cyanotic mucous membranes for several months was referred for ovariohysterectomy. Blood samples exhibited a noticeably brownish discoloration, while laboratory screening revealed mild-to-moderate erythrocytosis and near normal partial arterial oxygen pressure. Blood methemoglobin content was 41% of total hemoglobin concentration, and erythrocytic methemoglobin reductase activity was < 1% compared with control samples.

Hematologic, biochemical, hormonal, and redox status alterations during reproductive activity in adult rams of two breeds.

Background: Reproduction is a crucial, energy-consuming physiologic activity, which can temporarily disturb the homeostatic balance of the organism. Although rams are commonly placed in intense breeding programs on contemporary sheep farms, the clinicopathologic and redox status alterations have not been adequately studied during reproductive activity.

Objectives: The objective was to investigate the clinicopathologic and redox status changes in rams during reproductive activity.

Canine and feline dirofilariosis in a highly enzootic area: first report of feline dirofilariosis in Greece.

Dirofilaria immitis (heartworm) is enzootic in many areas of the world and quite prevalent in southern European countries. Although dogs are the main host of the parasite, cats may also be infected, and the prevalence of feline dirofilariosis is associated with the respective prevalence of canine infection in any given area. The aim of the present study was to investigate the proportion of D.

Whole-genome sequencing of a CTX-M-11-encoding and quinolone-non-susceptible Klebsiella pneumoniae ST194 isolate from a hospitalised dog in Greece.

Objectives: The emergence and spread of transferable β-lactamases among Enterobacteriaceae is a major problem both to human and veterinary medicine and is an important contributing factor to the development of multidrug-resistant bacterial isolates. In the present study, whole-genome sequencing of a Klebsiella pneumoniae isolate (LKP817909) resistant to first- and second-generation cephalosporins and non-susceptible to fluoroquinolones, isolated from a urine sample of a hospitalised dog, was performed.

Methods: Genome sequencing was performed on an Illumina MiniSeq Sequencing System.

Combined and breed-specific RIs for hematologic, biochemical, and hormonal analytes in Chios and Florina adult rams.

Background: Clinical pathology investigations are essential to evaluate the health status and practice management of ruminants. Appropriate and accurate RIs are required for the interpretation of laboratory results. Despite substantial phenotypic differences and management-related disparities between male and female sheep, RIs in rams are sparse and limited by small reference populations and outdated methods.

Reference intervals for serum reactive oxygen metabolites, biological antioxidant potential, and oxidative stress index in adult rams.

OBJECTIVE To establish reference intervals for serum reactive oxygen metabolites (ROMs), biological antioxidant potential (BAP), and oxidative stress index (OSi) in adult rams by use of controlled preanalytic and analytic procedures. ANIMALS 123 healthy 1- to 4-year-old rams of 2 Greek breeds (Chios [n = 62] and Florina [61]). PROCEDURES 4 hours after rams were fed, a blood sample was obtained from each ram, and serum was harvested.

Nodular granulomatous glossitis as the sole clinical sign in canine leishmaniosis.

A 5.5-year-old, intact male Rottweiler dog was admitted with a history of multifocal nodular tongue lesions which progressively deteriorated during the previous year. Physical examination revealed several reddish nodules with central depression on the surface of the tongue in an otherwise healthy dog.