LC-determination of five paraben preservatives in saliva and toothpaste samples using UV detection and a short monolithic column.

The present study reports the development and application of an HPLC-UV method for the simultaneous separation and determination of five paraben preservatives (methyl-, ethyl-, propyl-, n-butyl- and iso-butyl-paraben) in real samples. All analytes were separated efficiently in less than 20 min using a simple H(2)O:ACN linear gradient and a short monolithic column (50 mm x 4.6mm i.