Background: We describe a new surgical technique of in situ intestinal ischemia-reperfusion injury in the pig, which includes transection of the small bowel, extrinsic autonomic denervation, lymphatic disruption, and finally in-situ cold ischemia of the graft by flushing through the first jejunal artery.
Material And Methods: Ten female pigs were used for the study. All neural and lymphatic connections to the jejunoileum were transected.