Transcriptional Analysis of Total CD8 T Cells and CD8CD45RA Memory T Cells From Young and Old Healthy Blood Donors.

Memory CD8 T cells accumulate with aging, while the naïve T cell compartment decreases, leading to an increased susceptibility to infections and a decreased vaccine efficiency. To get deeper insights into the underlying mechanisms, this study aims to determine the age-dependent expression profile of total versus memory CD8 T cells from young and old donors. Total CD8 and CD8CD45RA memory T cells isolated from young (<30 years) and old (>60 years) donors were stimulated with anti-CD3 and anti-CD28 antibodies for 48h before analyzing the cytokine secretion and activation markers by flow cytometry and changes in the expression profiles using RNA sequencing.

A Detailed Analysis of the Locus Suggests a New Mechanism for Bolting after Winter in Sugar Beet ( L.).

Sugar beet ( ssp. vulgaris) is a biennial, sucrose-storing plant, which is mainly cultivated as a spring crop and harvested in the vegetative stage before winter. For increasing beet yield, over-winter cultivation would be advantageous.