The role of DNA demethylation in liver to pancreas transdifferentiation.

Background: Insulin producing cells generated by liver cell transdifferentiation, could serve as an attractive source for regenerative medicine. The present study assesses the relationship between DNA methylation pTFs induced liver to pancreas transdifferentiation.

Results: The transdifferentiation process is associated with DNA demethylation, mainly at gene regulatory sites, and with increased expression of these genes.

Knock-down of and additively extends lifespan and healthspan in .

Genetic manipulations can ameliorate the aging process and extend the lifespan of model organisms. The aim of this research was to identify novel genetic interventions that promote both lifespan and healthspan, by combining the effects of multiple longevity-associated gene inactivations in . For this, the individual and combined effects of the mutation and of and knock-downs were studied, both in the wild type and mutant backgrounds.

Phenotypic assessment of liver-derived cell cultures during expansion.

Liver cells represent an attractive source of cells for autologous regenerative medicine. The present study assesses the liver cells' stability during expansion, as a prerequisite for therapeutic use. The human liver cell cultures in this study were propagated efficiently for at least 12 passages.

The effect of liver donors' age, gender and metabolic state on pancreatic lineage activation.

Autologous cells replacement therapy by liver to pancreas transdifferentiation (TD) allows diabetic patients to be also the donors of their own therapeutic tissue. To analyze whether the efficiency of the process is affected by liver donors' heterogeneity with regard to age, gender and the metabolic state.  TD of liver cells derived from nondiabetic and diabetic donors at different ages was characterized at molecular and cellular levels, .

Hydrogen sulfide enhances pancreatic β-cell differentiation from human tooth under normal and glucotoxic conditions.

Aim: Glucotoxicity obstructs pancreatic differentiation from adult stem cells. The aim was to develop a novel protocol for differentiation of dental pulp stem cells (DPSCs) into pancreatic β cells and determine the effect of HS on glucotoxicity.

Materials & Methods: DPSCs were differentiated with media containing 5.