Material-independent film formation and autonomous degradation of Cu-tetrahydroxy-1,4-benzoquinone metal-organic complexes.

Metal-organic complexes (MOCs) have extensively been studied as prominent components in interface engineering. Once the designated missions of MOC films are achieved, or while they are still operational, it is preferred that the films undergo degradation on demand in certain circumstances. Current research on MOC-film degradation predominantly relies on chemical treatment, which can alter the states and conditions of specific systems.

Biocompatible hydrogel coating on single living cells through visible light-induced polymerization.

Visible light-mediated photocatalysis leads to the efficient hydrogel coating of individual mammalian cells, functionalized with biocompatible anchor molecules tagged with fluorescein serving as a trifecta: photocatalyst, initiator, and fluorophore. NIH3T3 fibroblast cells are encapsulated within hydrogel shells of poly(ethylene glycol) diacrylate (PEGDA) and -vinylpyrrolidone without any noticeable decrease in cell viability.

Construction of Liposome-Based Extracellular Artificial Organelles on Individual Living Cells.

Integration of living cells with extrinsic functional entities gives rise to bioaugmented nanobiohybrids, which hold tremendous potential across diverse fields such as cell therapy, biocatalysis, and cell robotics. This study presents a biocompatible method for incorporating multilayered functional liposomes onto the cell surface, creating extracellular artificial organelles or exorganelles. The introduction of various extrinsic functionalities to cells is achieved without comprising their viabilities.

Biphasic water-oil systems for functional augmentation of probiotic nanoencapsulated in luteolin-Fe shells.

Single-cell nanoencapsulation (SCNE) has great potential in the enhancement of therapeutic effects of probiotic microbes. However, the material scope has been limited to water-soluble compounds to avoid non-biocompatible organic solvents that are harmful to living cells. In this work, the SCNE of probiotic with water-insoluble luteolin and Fe ions is achieved by the vortex-assisted, biphasic water-oil system.

MolNet_Equi: A Chemically Intuitive, Rotation-Equivariant Graph Neural Network.

Although deep-learning (DL) models suggest unprecedented prediction capabilities in tackling various chemical problems, their demonstrated tasks have so far been limited to the scalar properties including the magnitude of vectorial properties, such as molecular dipole moments. A rotation-equivariant MolNet_Equi model, proposed in this paper, understands and recognizes the molecular rotation in the 3D Euclidean space, and exhibits the ability to predict directional dipole moments in the rotation-sensitive mode, as well as showing superior performance for the prediction of scalar properties. Three consecutive operations of molecular rotation , dipole-moment prediction , and dipole-moment inverse-rotation do not alter the original prediction of the total dipole moment of a molecule , assuring the rotational equivariance of MolNet_Equi.

A Micrometric Transformer: Compositional Nanoshell Transformation of Fe -Trimesic-Acid Complex with Concomitant Payload Release in Cell-in-Catalytic-Shell Nanobiohybrids.

Nanoencapsulation of living cells within artificial shells is a powerful approach for augmenting the inherent capacity of cells and enabling the acquisition of extrinsic functions. However, the current state of the field requires the development of nanoshells that can dynamically sense and adapt to environmental changes by undergoing transformations in form and composition. This paper reports the compositional transformation of an enzyme-embedded nanoshell of Fe -trimesic acid complex to an iron phosphate shell in phosphate-containing media.

Tandem-biocatalysis reactors constructed by topological evolution of CaCO particles into hollow metal hydroxide spheres.

Despite remarkable advances in the design and synthesis of hollow inorganic spheres (HISs), the harsh synthetic conditions have precluded the applications of HISs to biochemical and biological fields. Herein we report a biocompatible strategy for synthesizing metal hydroxide HISs (MH-HISs) by simply mixing CaCO particles with metal ions in water. The ion-exchange reaction between Ca and metal ions leads to the structural and chemical evolution from solid CaCO particles to hollow MH-HISs via core-shell and yolk-shell structures, while enabling the encapsulation of enzymes to the shells without loss of catalytic activities.

Fugetaxis of Cell-in-Catalytic-Coat Nanobiohybrids in Glucose Gradients.

Manipulation and control of cell chemotaxis remain an underexplored territory despite vast potential in various fields, such as cytotherapeutics, sensors, and even cell robots. Herein is achieved the chemical control over chemotactic movement and direction of Jurkat T cells, as a representative model, by the construction of cell-in-catalytic-coat structures in single-cell nanoencapsulation. Armed with the catalytic power of glucose oxidase (GOx) in the artificial coat, the nanobiohybrid cytostructures, denoted as Jurkat , exhibit controllable, redirected chemotactic movement in response to d-glucose gradients, in the opposite direction to the positive-chemotaxis direction of naïve, uncoated Jurkat cells in the same gradients.

A Natural Virucidal and Microbicidal Spray Based on Polyphenol-Iron Sols.

Numerous disinfection methods have been developed to reduce the transmission of infectious diseases that threaten human health. However, it still remains elusively challenging to develop eco-friendly and cost-effective methods that deactivate a wide range of pathogens, from viruses to bacteria and fungi, without doing any harm to humans or the environment. Herein we report a natural spraying protocol, based on a water-dispersible supramolecular sol of nature-derived tannic acid (TA) and Fe, which is easy-to-use and low-cost.

Neutralization of Cannabidiol Neurotoxicity in Neuron-Astrocyte Sandwich Coculture.

Cannabidiol (CBD), a main nonpsychoactive phytocannabinoid in the Cannabis genus, has been in the limelight for its potential health benefits in various neurological diseases. However, the safety issue of CBD in the nervous system has not been settled fully, while CBD has been reported to have mild side effects including dizziness and somnolence. In this work, a platform of neuron-astrocyte sandwich coculture to investigate the neurotoxicity of CBD, as well as the neuronal responses to CBD, in a more in vivo relevant mode is constructed.

Vortex-assisted, nanoarchitectonic manipulation of microparticles with flavonoid-Fe complex in biphasic water-oil systems.

Coordination-driven self-assembly of metal-ligand complexes is a powerful nanoarchitectonic tool for particle engineering, but its usability is limited when using two immiscible coating components. This paper reports that simple vortexing of a biphasic system of Fe ions in water and flavonoids in oil forms nanoshells on individual particles, thereby enabling the utilization of water-insoluble ligands as coating materials. Mechanistic studies suggest that the biphasic mass-transfer equilibrium of flavonoid-Fe species controls the shell formation, with the oil phase acting as a reservoir of coating precursors for continuous coating.

Enzyme-Mediated Kinetic Control of Fe-Tannic Acid Complexation for Interface Engineering.

Supramolecular self-assembly of Fe and tannic acid (TA) has received great attention in the fields of materials science and interface engineering because of its exceptional surface coating properties. Although advances in coating strategies often suggest that kinetics in the generation of interface-active Fe-TA species is deeply involved in the film formation, there is no acceptable elucidation for the coating process. In this work, we developed the enzyme-mediated kinetic control of Fe oxidation to Fe in a Fe-TA complex in the iron-gall-ink-revisited coating method.

Rotational Variance-Based Data Augmentation in 3D Graph Convolutional Network.

This work proposes the data augmentation by molecular rotation, with consideration that the protein-ligand binding events are rotation-variant. As a proof-of-concept, known active (i. e.

A Decade of Advances in Single-Cell Nanocoating for Mammalian Cells.

Strategic advances in the single-cell nanocoating of mammalian cells have noticeably been made during the last decade, and many potential applications have been demonstrated. Various cell-coating strategies have been proposed via adaptation of reported methods in the surface sciences and/or materials identification that ensure the sustainability of labile mammalian cells during chemical manipulation. Here an overview of the methodological development and potential applications to the healthcare sector in the nanocoating of mammalian cells made during the last decade is provided.

Neuroprotective Effect of Cannabidiol Against Hydrogen Peroxide in Hippocampal Neuron Culture.

Reports on the neurotoxic and neuroprotective effects of cannabidiol (CBD) have not been in complete accord, showing different and somewhat contradictory results depending upon the brain cell types and experimental conditions employed. This work systematically examines the neuroprotective capability of CBD against oxidative stress (i.e.

Inexpensive water soluble methyl methacrylate-functionalized hydroxyphthalimide: variations of the mycophenolic acid core for selective live cell imaging of free cysteine.

Evident from numerous studies, cysteine plays a crucial role in cellular function. Reactions with analyte also enables for molecular recognition to adhere to molecular therapeutic potential; integration between synthetic probes therefore allows for a potentially deep therapy-related interogation of biological systems (theranostics). The development of molecular cysteine probes with extremely accurate detection is still a key challenge for the field.

Reversed Anionic Hofmeister Effect in Metal-Phenolic-Based Film Formation.

Although metal-phenolic species have emerged as one of the versatile material-independent-coating materials, providing attractive tools for interface engineering, mechanistic understanding of their film formation and growth still remains largely unexplored. Especially, the anions have been overlooked despite their high concentration in the coating solution. Considering that the anions are critical in the reactivity of metal-organic complex and the formation and/or property of functional materials, we investigated the anionic effects on the characteristics of film formation, such as film thickness and properties, in the Fe-tannic acid coating.

Layer-wise relevance propagation of InteractionNet explains protein-ligand interactions at the atom level.

Development of deep-learning models for intermolecular noncovalent (NC) interactions between proteins and ligands has great potential in the chemical and pharmaceutical tasks, including structure-activity relationship and drug design. It still remains an open question how to convert the three-dimensional, structural information of a protein-ligand complex into a graph representation in the graph neural networks (GNNs). It is also difficult to know whether a trained GNN model learns the NC interactions properly.

Ascorbic acid-mediated reductive disassembly of Fe-tannic acid shells in degradable single-cell nanoencapsulation.

Rapid degradation of Fe-tannic acid films is achieved under mild conditions via ascorbic acid-mediated Fe reduction, which overcomes the problems in the disassembly of a metal-organic complex including slow reaction rates and reaction incompatibility with living cells. The strategy of reductive disassembly is applied to degradable single-cell nanoencapsulation, providing an advanced tool for tightly controlling and manipulating the cell-material interface.