Application of RAPD and SCAR markers for purity testing of F1 hybrid seed in chili pepper (Capsicum annuum).

A simpler and better method for purity testing of hybrid pepper seed was developed. The simplest method for extracting genomic DNA, the NaOH method, was chosen. Two RAPD markers identifying male and female parents were also developed, and the PCR products of male- and female-specific RAPD markers were cloned and sequenced.