RECENT FOCUS IN NON-SELEX-COMPUTATIONAL APPROACH FOR DE NOVO APTAMER DESIGN: A MINI REVIEW.

Aptamers, single-stranded nucleic acids that bind to specific targets with high affinity and specificity, hold significant promise in various biomedical and biotechnological applications. The traditional method of aptamer selection, SELEX (Systematic Evolution of Ligands by EXponential Enrichment) takes a lot of work and time. Recent advancements in computational methods have revolutionized aptamer design, offering efficient and effective alternatives.

Green synthesis of copper nanoparticles: a promising solution for drug resistance and cancer therapy challenges.

Green synthesis techniques have drawn a lot of interest lately since they are beneficial to the environment and have potential uses in a variety of industries, including biomedicine. Because of their special physicochemical characteristics, copper nanoparticles (CuNPs) have become one of the most interesting options for use in biological applications among nanomaterials. An overview of green synthesis methods for CuNPs is given in this review, along with a discussion of their applications in cancer therapeutics.

Synthesis of novel spiroisoxazolidino hybrids of alantolactone and isoalantolactone via 1,3 dipolar nitrone cycloaddition and its antimicrobial Evaluation.

Alantolactone and isoalantolactone are two isomeric sesquiterpene lactones that were isolated from Innula recemosa. Here, we are used for the semisynthesis of novel isoxazolidine hybrids of alantolactone and isoalantolactone through a two-step process: nitrone synthesis followed by nitrone 1,3-dipolar cycloaddition. The formation of the cycloadduct was well characterized via modern spectroscopic techniques such as HRMS, H NMR, C NMR, DEPT-90, DEPT-135, and 2D NMR.

Enhancement of detoxification of xenobiotic aromatic amine dyes by N-acetyltransferase 1 (NAT1) enzyme on human keratinocytes cells through structural modification.

The metabolic conversion of aromatic amines to N-acetylated forms in skin and keratinocytes depends on N-acetyltransferase-1 (NAT1). Common hair color ingredient such as para-phenylenediamine (PPD) causes allergic contact dermatitis. We explored how different electronic substituents on PPD aided NAT1 enzyme biotransform oxidative arylamine (AA) compounds G1-G13 by N-acetylation, NAT-1 activity assays, metabolism, and in vitro clearance investigations in human keratinocytes, while identifying NAT-1 protein levels by Western blot and qRT-PCR.

The Isolation and Assessment of the Levels of Microbial Pathogens From Saliva, Hands, and Currency Notes.

Introduction Currencies such as coins and paper notes are always used for the purchase of products and services by the common man. These currencies are constantly being subjected to contamination and thus serve as a potential medium for the transfer of microbial pathogens. Thus, this study aims to establish public awareness of the proper handling of currency notes to prevent cross-contamination and the incidence of infectious diseases.

Prevalence of economic violence among the geriatric population and their knowledge and utilization of social security schemes in Chengalpattu district, Tamil Nadu.

Context: Economic independence is an important factor in the prevention of violence among the elderly. Optimal utilization of various social security schemes in India helps them in achieving financial independence and proper utilization of health care services. However, there is a knowledge and service utilization gap among the elderly in our population.

Serum megalin levels in type-2 diabetes mellitus with and without cardiovascular disease.

Background: Low-density lipoprotein receptor-related protein-2 (LRP2), also called megalin, is a multi-ligand receptor of the LDL receptor gene family mediating reabsorption of ligands like Apo-A1. Type 2 diabetes mellitus (T2DM) may possibly disrupt megalin functions as it is found to be regulated by insulin. This might cause cardiovascular complications due to derangement in lipoprotein metabolism.

'Finally making sense': graphic medicine and ADHD diagnosis in adulthood.

This article aims to examine the lived experiences of attention-deficit/hyperactivity disorder (ADHD) diagnosis in adulthood, emphasising its revelatory nature and diverse emotional responses it provokes. The diagnosis serves as a pivotal moment of self-discovery, often evoking feelings of validation and identity affirmation. However, it also triggers a complex array of emotions, including grieving for the childhood self, frustration with society's failure to recognise the legitimate challenges and evolving self-concept post diagnosis.

Trichuriasis in Human Patients from Côte d'Ivoire Caused by Novel Trichuris incognita Species with Low Sensitivity to Albendazole/Ivermectin Combination Treatment.

Albendazole/ivermectin combination therapy is a promising alternative to benzimidazole monotherapy alone for Trichuris trichiura control. We used fecal DNA metabarcoding to genetically characterize Trichuris spp. populations in patient samples from Côte d'Ivoire showing lower (egg reduction rate <70%) albendazole/ivermectin sensitivity than those from Laos and Tanzania (egg reduction rates >98%).

Cell- and tissue-specific glycosylation pathways informed by single-cell transcriptomics.

While single-cell studies have made significant impacts in various subfields of biology, they lag in the Glycosciences. To address this gap, we analyzed single-cell glycogene expressions in the Tabula Sapiens dataset of human tissues and cell types using a recent glycosylation-specific gene ontology (GlycoEnzOnto). At the median sequencing (count) depth, ∼40-50 out of 400 glycogenes were detected in individual cells.

Deciphering the role of exosomal derived regulatory noncoding RNAs as potential biomarkers for diabetic retinopathy: a systematic review.

About one-third of individuals with diabetes develop diabetic retinopathy (DR), with one-tenth experiencing vision-threatening conditions such as diabetic macular edema (DME) or proliferative diabetic retinopathy (PDR). Current treatments only show recovery in 50% of cases, and the disease often remains asymptomatic. Therefore, novel early detection methods and new biomarkers are crucial.

The effect of novel Head and Trunk Control Rehabilitation (HATCoRe) device in children with cerebral palsy: Single-Subject multiple baseline protocol.

Objective: Head and trunk control is essential for enhancing engagement and participation by improving visual integration, respiration, oromotor skill, arm control, and self-care. Our study protocol aims to investigate the effect of novel Head And Trunk Control Rehabilitation (HATCoRe) device on promoting head and trunk control in children with Cerebral Palsy (CP).

Method: This single subject multiple baseline design trial will include six children with CP aged two to 10 years, exhibiting delayed head and trunk control, having Gross Motor Function Classification System level IV and V will be enrolled.

Human Papilloma Virus Circulating Cell-Free DNA Kinetics in Cervical Cancer Patients Undergoing Definitive Chemoradiation.

Purpose: The human papillomavirus (HPV) is a significant cause of cervical cancer. We hypothesized that detecting viral cell-free HPV DNA (cfDNA) before, during, and after chemoradiation (chemoRT) could provide insights into disease extent, clinical staging, and treatment response.

Experimental Design: Sixty-six patients with locally advanced cervical cancer were enrolled between 2017 and 2023.

Functional constraints of killer meiotic drivers.

Killer meiotic drivers are selfish DNA loci that sabotage the gametes that do not inherit them from a driver+/driver- heterozygote. These drivers often employ toxic proteins that target essential cellular functions to cause the destruction of driver- gametes. Identifying the mechanisms of drivers can expand our understanding of infertility and reveal novel insights about the cellular functions targeted by drivers.

Duration of viral persistence in human semen after acute viral infection: a systematic review.

The persistence of viruses in human semen following acute infection can contribute to the ongoing transmission of a disease or cause resurgence after an outbreak has been declared ended. Viral persistence in semen affects embryonic development and male fertility, and the development of drugs and vaccines. We conducted a systematic review of viral persistence in semen in accordance with PRISMA guidelines.

Sulfur-functionalized sawdust biochar for enhanced cadmium adsorption and environmental remediation: A multidisciplinary approach and density functional theory insights.

Pristine biochar typically exhibits limited capacity for heavy metal adsorption due to its inadequate pore development and insufficient surface functionality. This study introduces an innovative chemical strategy to enhance the surface of sawdust biochar with sulfur-based functional groups (C=S, C-S, S-S, S, S-H, -SO, -SO) to significantly improve cadmium (Cd) adsorption. Sulfur-doping using HSO, NaS, and NaSO markedly increased the sulfur content from 0.

Biologically Enhanced Machine Learning Model to uncover Novel Gene-Drug Targets for Alzheimer's Disease.

Given the complexity and multifactorial nature of Alzheimer's disease, investigating potential drug-gene targets is imperative for developing effective therapies and advancing our understanding of the underlying mechanisms driving the disease. We present an explainable ML model that integrates the role and impact of gene interactions to drive the genomic variant feature selection. The model leverages both the Alzheimer's knowledge base and the Drug-Gene interaction database (DGIdb) to identify a list of biologically plausible novel gene-drug targets for further investigation.

Corrigendum to "Empowerment of Primary Healthcare Providers on the Prevention and Management of Dental or Oral Health Issues among Postchemotherapy Patients in Pandemic".

[This corrects the article DOI: 10.1155/2022/9087776.].

Meta-analysis on Effectiveness of Palliative Care versus Conventional Care amongst Advanced Gynaecological Patients with Cancer and Caregivers.

In cancer patients, physical and psychological issues are very common. There is a need for family support and high utilisation of healthcare resources commonly. Palliative care (PC) has grown in popularity to better fulfil of needs of patients and their families and potentially lowering hospital costs.

Enhancing cherry tomato packaging: Evaluation of physicochemical, mechanical, and antibacterial properties in tannic acid and gallic acid crosslinked cellulose/chitosan blend films.

The aim of this work was to study the characteristics of composites fabricated from cellulose (CL) and chitosan (CS) blends that were reinforced with different tannic acid (TA) and gallic acid (GA) concentrations. The structure and antibacterial activity of CL/CS composite films were investigated with the addition of TA and GA. The composite films were subjected to mechanical, water contact angle (WCA), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Scanning electron microscopy (SEM), and antibacterial activity tests.

Palladium Nanoparticle-Decorated Copper-Hemin Metal Organic Framework for Enzymatic Electrochemical Detection of Creatinine in Human Urine.

Creatinine is indeed a crucial biomarker for kidney diseases. In this work, a novel electrochemical biosensor based on a copper-hemin metal organic framework [Cu-hemin metal-organic framework (MOF)] nanoflake decorated with palladium (Pd) (Pd/Cu-hemin MOF) was fabricated and incorporated with creatinine deiminase (CD) on a glassy carbon electrode (GCE) for creatinine detection. The formation of a Pd/Cu-hemin MOF composite was confirmed by X-ray photoelectron spectroscopy, X-ray diffraction, and Fourier transform infrared spectroscopy.