Development and validation of a microarray for the confirmation and typing of norovirus RT-PCR products.

Noroviruses are implicated in many worldwide institutional, food and waterborne outbreaks each year. Genetic typing of isolates is valuable for monitoring outbreak spread as well as variation in circulating strains. Microarrays have the potential to provide rapid genotype information for norovirus samples.

Influence of alginic acid and fucoidan on the immune responses of head kidney leukocytes in cod.

The present study investigated the immunomodulatory activities of alginic acid and fucoidan, both derived from brown seaweeds, on selected cellular immune responses and antibacterial activity of head kidney (HK) leukocytes of cod, Gadus morhua. Primary cultures of HK leukocytes were incubated with either 10 or 100 μg ml⁻¹ of the substances and the effects on respiratory burst, cellular proliferation, acid and alkaline phosphatase activity and cellular myeloperoxidase were measured at 3- and 24-h post-incubation. The antibacterial activity of the supernatants collected from the cell cultures incubated with 100 μg ml⁻¹ of the substances were tested against Vibrio anguillarum and Aeromonas salmonicida.

In vivo modulation of immune response and antioxidant defense in Atlantic cod, Gadus morhua following oral administration of oxolinic acid and florfenicol.

Oxolinic acid and florfenicol are the commonly used antibiotics for the treatment of bacterial diseases in Atlantic cod, Gadus morhua. The changes in selected innate humoral immune response of the fish, bacterial proliferation in serum and transcriptional activity of selected immune and antioxidant defense-related genes following oral administration of these antimicrobial compounds were evaluated. Juvenile cod (75-100 g) were fed commercial feed coated with either florfenicol (10 mg kg(-1) fish, active ingredient) or oxolinic acid (20 mg kg(-1) fish, active ingredient) at a ration of 0.

Bovine CD2-/NKp46+ cells are fully functional natural killer cells with a high activation status.

Background: Natural killer (NK) cells in the cow have been elusive due to the lack of specific NK cell markers, and various criteria including a CD3-/CD2+ phenotype have been used to identify such cells. The recent characterization of the NK-specific NKp46 receptor has allowed a more precise definition of bovine NK cells. NK cells are known as a heterogeneous cell group, and we here report the first functional study of bovine NK cell subsets, based on the expression of CD2.

Evaluation of the gamma interferon test for diagnosis of paratuberculosis in goats.

The gamma interferon assay was evaluated for diagnosis of paratuberculosis in goats with special emphasis on false positive reactions. Four categories of herds were tested: (A) herds that had a history of paratuberculosis, had given positive Mycobacterium avium subsp. paratuberculosis fecal samples and were vaccinated against paratuberculosis; (B) herds that had been vaccinated but had never shown clinical signs of paratuberculosis nor given positive M.

NKp46 defines a subset of bovine leukocytes with natural killer cell characteristics.

Natural killer (NK) cells have not previously been precisely identified or characterized in cattle or any other ruminant species. We have generated a monoclonal antibody against bovine NKp46, which is expressed exclusively by NK cells in man. NKp46+ cells comprised 1-10% of blood mononuclear cells in cattle, and did not stain with antibodies against CD3, CD4, TCR1, B cell or granulocyte markers.