Incorporating a liquid-core-waveguide cell in recycling liquid chromatography for detailed studies of photodegradation reactions.

In this work, a microfluidic photoreactor was embedded in a recycling liquid-chromatography system. Mixtures were separated on an analytical column and compounds of interest were subsequently introduced into the light-reactor cell. After degradation, the content of the light-reactor cell was reinjected onto the same column to separate the parent compound from its degradation products.

Liquid Core Waveguide Cell with In Situ Absorbance Spectroscopy and Coupled to Liquid Chromatography for Studying Light-Induced Degradation.

In many areas, studying photostability or the mechanism of photodegradation is of high importance. Conventional methods to do so can be rather time-consuming, laborious, and prone to experimental errors. In this paper we evaluate an integrated and fully automated system for the study of light-induced degradation, comprising a liquid handler, an irradiation source and exposure cell with dedicated optics and spectrograph, and a liquid chromatography (LC) system.