Publications by authors named "Ingrid de Siqueira Pereira"
Extracellular Vesicles from Contribute in Stimulating Immune Response and Immunosuppression in Hosts with Visceral Leishmaniasis.
Microorganisms
January 2024
Article Synopsis
- Visceral leishmaniasis (VL) is a chronic disease in Brazil caused by specific parasites, with extracellular vesicles (Leish-EVs) playing a role in how the host immune system responds to the infection.
- The study found that Leish-EVs are released more when the parasites grow at higher temperatures, and the presence of these vesicles in the host cells can help differentiate between infected and uninfected individuals.
- THP-1 cells exposed to Leish-EVs showed altered immune responses, producing certain interleukins and microRNAs, indicating that Leish-EVs could contribute to immunosuppression and worsen the disease in infected hosts.
Toxoplasmosis causes serious harm to the fetus, as tachyzoite dissemination, during pregnancy in women developing the primo-infection. The microRNAs (miRNAs) are small non-coding RNAs, which have regulatory roles in cells by silencing messenger RNA. Circulating miRNA are promising biomarkers for diagnosis and prognosis of numerous diseases.
View Article and Find Full Text PDFThis retrospective cohort study analysed extracellular vesicles (EVs) and microRNAs (miRNAs) excreted in canine sera from dogs with canine visceral leishmaniasis (CanVL). A total of 56 canine sera were divided into Group I (28, from healthy dogs) and Group II (28, from the same dogs, but already with CanVL). CanVL was determined by clinical and laboratory diagnoses.
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- This study analyzed extracellular vesicles (EVs) from mouse sera infected with Toxoplasma gondii or immunized with EVs derived from the parasite, identifying differences in EV concentrations and types across different mouse groups.
- EVs were primarily microvesicles in infected groups (acute and chronic), while normal mice had significantly lower concentrations, confirmed by electron microscopy.
- Immunological responses varied, with certain cytokines and miRNAs being up-regulated in immunized and chronically infected mice compared to normal mice, suggesting that EV concentration could differentiate infected from non-infected hosts.
Combining urine antigen and blood polymerase chain reaction for the diagnosis of disseminated histoplasmosis in hospitalized patients with advanced HIV disease.
Med Mycol
September 2021
Unlabelled: Disseminated histoplasmosis (DH) is endemic in Latin America and the Caribbean where diagnostic tools are restricted. We carried-out a 1-year prospective cohort study at a referral hospital in São Paulo, Brazil. Participants had > or =18 years old, were hospitalized due to any indication and had CD4+ < 200 cells/µl.
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- The study focused on analyzing extracellular vesicles (EVs), specifically microvesicles and exosomes, found in serum and cerebrospinal fluid (CSF) of patients affected by cerebral or gestational toxoplasmosis.
- Researchers examined clinical samples from 83 individuals divided into four groups based on their toxoplasmosis status and HIV infection to compare concentrations and characteristics of EVs.
- Findings revealed that serum-derived EVs from toxoplasmosis patients had significantly higher concentrations compared to healthy individuals, while the size of EVs was consistent across groups, indicating concentration rather than size could differentiate toxoplasmosis patients from non-infected individuals.
Ocular toxoplasmosis associated with up-regulation of miR-155-5p/miR-29c-3p and down-regulation of miR-21-5p/miR-125b-5p.
Cytokine
March 2020
Ocular toxoplasmosis (OT) is one of the most common manifestations of Toxoplasma gondii infection and can be related with congenital or acquired infections. OT cause posterior uveitis that cause serious sequelae as complete loss of vision. microRNAs (miRNAs) are small non-coding RNAs, which have regulatory roles in cells by silencing messenger RNA.
View Article and Find Full Text PDFThis study investigated the potential of five miRNA candidates for cerebral toxoplasmosis/HIV co-infection (CT/HIV) biomarkers. miR-155-5p, miR-146a-5p, miR-21-5p, miR-125b-5p and miR-29c-3p were tested in 79 plasma divided into groups: 32 CT/HIV patients; 27 individuals with asymptomatic toxoplasmosis (AT); and 20 individuals seronegative for toxoplasmosis (NC). From each was collected peripheral blood/EDTA for laboratory diagnosis.
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