Publications by authors named "Ingrid E Ehrmann"
Article Synopsis
- RBMXL2, a nuclear protein specific to germ cells, represses cryptic splicing during meiosis and is crucial for male fertility, while its similar counterpart RBMX regulates splicing in somatic cells.
- RBMX interacts with long exons and helps maintain genome stability by preventing the use of harmful splice sites, complementing the gene silencing that occurs during male meiosis.
- Both RBMX and RBMXL2 share parallel functions across somatic and germline tissues, suggesting their roles in splicing have been evolutionarily conserved for over 200 million years.
RBMY is a male germline RNA binding protein and potential alternative splicing regulator, but the lack of a convenient biological system has made its cellular functions elusive. We found that human RBMY fused to green fluorescent protein was strictly nuclear in transfected cells, but spatially enriched in areas around nuclear speckles with some components of the exon junction complex (EJC). Human RBMY (hRBMY) and the EJC components Magoh and Y14 also physically interacted but, unlike these two proteins, hRBMY protein did not shuttle to the cytoplasm.
View Article and Find Full Text PDFMale gametes originate from a small population of spermatogonial stem cells (SSCs). These cells are believed to divide infinitely and to support spermatogenesis throughout life in the male. Here, we developed a strategy for the establishment of SSC lines from embryonic stem (ES) cells.
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